Identification of UGT85A glycosyltransferases associated with volatile conjugation in grapevine(Vitis vinifera × Vitis labrusca)  

作　　者：Xiangmei Cao Dan Jiang Hanqing Wang Boping Wu Jianhui Cheng Bo Zhang 

机构地区：[1]Laboratory of Fruit Quality Biology/Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Hangzhou,Zhejiang 310058,China [2]Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable,Ministry of Agriculture and Rural Affairs,College of Horticulture Science,Zhejiang A&F University,Hangzhou,Zhejiang 311300,China [3]Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021,China

出　　处：《Horticultural Plant Journal》2023年第6期1095-1107,共13页园艺学报（英文版）

基　　金：supported by the Key Project for New Agricultural Cultivar Breeding in Zhejiang Province (Grant No.2021C02066-6);China Postdoctoral Science Foundation (Grant No.2020M671732);the Fundamental Research Funds for the Zhejiang Provincial Universities (Grant No.2021XZZX026)。

摘　　要：Glycosylated volatiles are considered as hidden aroma precursors that affect fruit flavor and are involved in plant defense response. Several uridine-diphosphate(UDP)-glycosyltransferase(UGT) members related to the glycosylation of volatiles have been identified from grapevine berries. Although grapevine leaves produced much higher levels of glycosylated volatiles than berries, UGTs responsible for the production of glycosylated volatiles in leaves have not yet been identified. Here, eight novel UGT genes were cloned, among these four members that belong to UGT85A family(named UGT85A24, UGT85A25, UGT85A26, and UGT85A27) were localized in the cytoplasm and their recombinant proteins showed activity toward volatiles detected in grapevine, with geraniol as the preferred substrate in vitro. Two UGT genes with 98.1% homology differ in R/S-linalool selection. The UGT85A26-catalyzed substrate showed enrichment in S-linalool, whereas UGT85A27 showed a strong preference for R-linalool. UGT85A24 and UGT85A25 were expressed mainly in berries, and UGT85A26 and UGT85A27 were expressed predominantly in grapevine mature leaves and correlated with the highest content of glycosylated volatiles in the mature leaves. Methyl jasmonate(MeJA) induced the expression of UGT85A26 and UGT85A27 as well as the production of linalyl-β-glucoside, citronellyl-β-glucoside and phenethyl-β-glucoside. Transient overexpression of UGT85A26 and UGT85A27 in tobacco leaves led to the accumulation of linalyl-β-glucoside and an increase in citronellyl-β-glucoside in vivo. These results indicate that the expression of UGT85A26 and UGT85A27 is a major regulator affecting the content of glycosylated volatiles in grapevine.

关 键 词：UDP-glycosyltransferase Grape Volatiles Defense MEJA 

分 类 号：S663.1[农业科学—果树学]