布鲁氏菌Rev.1株接种不同条件制备TSA培养基验证试验  

Verification Test of Preparing TSA Medium by Inoculating Brucella Rev.1 Strain under Different Conditions

作　　者：吴梅花 狄栋栋 赵丹彤 白白音宝力高 侯振宇 WU Meihua;DI Dongdong;ZHAO Dantong;BAI Baiyinbaoligao;HOU Zhenyu(Jinyu Baoling Biopharmaceutical Co.,Ltd,Hohhot Inner Mongolia 010111,China)

机构地区：[1]金宇保灵生物药品有限公司,内蒙古呼和浩特010111

出　　处：《现代畜牧科技》2023年第12期32-34,共3页Modern Animal Husbandry Science & Technology

摘　　要：布鲁氏菌病是严重威胁人类健康的动物源性人畜共患病之一,动物布鲁氏菌病防治以预防为主。目前国内布鲁氏菌病疫苗有S2、A19、M5等,国际上还有S19、Rev.1等株疫苗使用,其中以羊种M5株和Rev.1株稳定性最不好。TSA是胰蛋白胨大豆琼脂培养基(也称胰酪大豆胨琼脂培养基),为商品化培养基,是布鲁氏菌Rev.1株适宜生长的培养基。选择不同琼脂浓度TSA培养基平板在不同条件(温度和时间)放置后接种布鲁氏菌Rev.1株,培养96 h后观察菌落生长情况并进行菌落结晶紫染色。通过对比分析发现,TSA培养基平板的湿度是影响变异检验结果准确性的重要因素,同时明确了布鲁氏菌Rev.1株所需TSA培养基的制备方法。Brucellosis is one of the zoonotic diseases of animal origin that seriously threatens human health.The prevention and control of brucellosis in animals is mainly based on prevention.At present,there are S2,A19 and M5 vaccines for brucellosis in China,and S19 and Rev.1 vaccines in the world,among which M5 and Rev.1 strains of sheep are the least stable.TSA is Tryptone soy agar medium(also known as Tryptone soy agar medium),a commercial medium that is suitable for the growth of Brucella Rev.1 strain.After TSA medium plate with different agar concentration was placed under different conditions(temperature and time),Brucella Rev.1 strain was inoculated.After 96 h culture,colony growth was observed and colony crystal violet staining was performed.Through comparative analysis,it is found that the humidity of TSA medium plate is an important factor affecting the accuracy of variation test results.At the same time,the preparation method of TSA medium for Brucella Rev.1 strain was clarified.

关 键 词：布鲁氏菌Rev.1株 琼脂浓度 温度和时间 变异率 

分 类 号：S855.12[农业科学—临床兽医学]