紫檀芪通过激活线粒体自噬减轻小鼠肾缺血再灌注损伤  被引量:2

Pterostilbene alleviates renal ischemia-reperfusion injury in mice by activating mitochondrial autophagy

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作  者:明浩 张云砚 梅胜兰 余艳丽[1] 孟庆涛[1] 夏中元[1] MING Hao;ZHANG Yunyan;MEI Shenglan;YU Yanli;MENG Qingtao;XIA Zhongyuan(Department of Anesthesiology,Renmin Hospital of Wuhan University,Wuhan,Hubei 430060,China)

机构地区:[1]武汉大学人民医院麻醉科,武汉430060

出  处:《重庆医学》2023年第23期3533-3537,共5页Chongqing medicine

摘  要:目的研究紫檀芪(PTE)对小鼠肾缺血再灌注损伤(IRI)的保护作用及相关机制。方法将24只C57小鼠分成4组(n=6),包括假手术组(S组)、肾缺血再灌注组(IR组)、肾缺血再灌注+5 mg/kg PTE组(IR+PTE1组)和肾缺血再灌注+10 mg/kg PTE组(IR+PTE2组)。采用苏木素-伊红(HE)及TUNEL染色评估肾组织损伤。采用相关试剂盒检测血清肌酐(Cr)、尿素氮(BUN)、丙二醛(MDA)、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和IL-6水平。采用Western blot检测肾脏组织Bcl-2相互作用蛋白3(BNIP3)和微管相关蛋白轻链(LC)-3Ⅱ的表达。结果与S组比较,IR组血清Cr、BUN、MDA、TNF-α、IL-1β、IL-6水平升高,肾小管损伤评分及凋亡指数升高,BNIP3和LC3-Ⅱ表达下调,差异有统计学意义(P<0.05);与IR组比较,IR+PTE1组血清Cr、BUN、MDA、TNF-α、IL-1β、IL-6水平降低,肾小管损伤评分及凋亡指数降低,BNIP3和LC3-Ⅱ表达上调,差异有统计学意义(P<0.05);与IR+PTE1组比较,IR+PTE2组血清Cr、BUN、MDA、TNF-α、IL-1β、IL-6水平降低,肾小管损伤评分及凋亡指数降低,BNIP3和LC3-Ⅱ表达上调,差异有统计学意义(P<0.05)。结论PTE对小鼠肾IRI具有保护作用。Objective To study the protective effect and related mechanism of pterostilbene(PTE)on renal ischemia-reperfusion injury(IRI)in mice.Methods Twenty-four C57 mice were randomly divided into 4 groups(n=6),including the sham operation group(S group),the renal ischemia reperfusion group(IR group),the renal ischemia reperfusion+5 mg/kg PTE group(IR+PTE1 group)and the renal ischemia reperfusion+10 mg/kg PTE group(IR+PTE2 group).Hematoxylin-eosin(HE)and TUNEL staining were used to evaluate renal tissue injury.Creatinine(Cr),blood urea nitrogen(BUN),malondialdehyde(MDA)in renal tissue,inflammatory cytokines tumor necrosis factor(TNF-α),interleukin(IL)-1βand IL-6 levels in serum were detected with relevant kits.The expression of Bcl-2 interacting protein 3(BNIP3)and microtubule-associated protein light chain(LC)-3Ⅱin kidney tissue was detected by Western blot.Results Compared with the S group,serum levels of Cr,BUN,MDA,TNF-α,IL-1βand IL-6 in the IR group were increased,renal tubule injury score and apoptosis index were increased,and expressions of BNIP3 and LC-3Ⅱwere down-regulated,with statistical significance(P<0.05).Compared with the IR group,serum levels of Cr,BUN,MDA,TNF-α,IL-1βand IL-6 in the IR+PTE1 group were decreased,renal tubule injury score and apoptosis index were decreased,and expressions of BNIP3 and LC-3Ⅱwere up-regulated,with statistical significance(P<0.05).Compared with the IR+PTE1 group,serum levels of Cr,BUN,MDA,TNF-α,IL-1βand IL-6 in the IR+PTE2 group were decreased,renal tubule injury score and apoptosis index were decreased,and expressions of BNIP3 and LC-3Ⅱwere up-regulated,with statistical significance(P<0.05).Conclusion PTE has protective effect on renal IRI in mice.

关 键 词:缺血再灌注 紫檀芪 线粒体自噬 氧化应激 肾脏 

分 类 号:R34[医药卫生—基础医学]

 

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