CD19^(+)CD24^(hi)CD38^(hi)调节性B淋巴细胞在妊娠期高血压疾病中的异常表达分析  

Abnormal expression of CD19^(+)CD24^(hi)CD38^(hi)B lymphocytes in hypertensive disorder complicating pregnancy

作　　者：何花 李诗雨 高鹰[1] 杨林[1] 任为[1] HE Hua;LI Shiyu;GAO Ying;YANG Lin;Ren wei(Department of Obstetrics,Maternal and Child Health Hospital of Hubei Province,Wuhan,Hubei 430070,China)

机构地区：[1]湖北省妇幼保健院产科,武汉430070

出　　处：《重庆医学》2023年第23期3598-3602,共5页Chongqing medicine

基　　金：湖北省卫生健康委员会联合基金项目(WJ2019H298)。

摘　　要：目的探讨CD19^(+)CD24^(hi)CD38^(hi)调节性B淋巴细胞及其分泌的细胞因子白细胞介素-10(IL-10)在妊娠期高血压疾病中的表达及意义。方法选取该院2020年9月至2021年5月收治的15例妊娠期高血压患者(妊娠期高血压组)、11例子痫前期患者(子痫前期组),另选取同期12例正常妊娠孕妇(对照组)。应用流式细胞术分别检测3组孕妇终止妊娠前外周血CD19^(+)B淋巴细胞、CD19^(+)CD24^(hi)CD38^(hi)调节性B淋巴细胞及IL-10的表达。结果3组血清CD19+B淋巴细胞百分比比较,差异无统计学意义(P>0.05)。3组血清CD19^(+)CD24^(hi)CD38^(hi)调节性B淋巴细胞百分比两两比较,对照组>妊娠期高血压组>子痫前期组,且对照组与妊娠期高血压组、子痫前期组比较,差异均有统计学意义(P<0.05)。3组血清IL-10表达水平两两比较,对照组>妊娠期高血压组>子痫前期组,差异均有统计学意义(P<0.05)。结论CD19^(+)CD24^(hi)CD38^(hi)调节性B淋巴细胞及其分泌的IL-10在妊娠期高血压疾病孕妇中异常表达,可能与妊娠期高血压疾病的发生、发展相关。Objective To investigate the expression and significance of CD19^(+)CD24^(hi)CD38^(hi)regulatory B lymphocytes and secreted cytokine interleukin-10(IL-10)in hypertensive disorder complicating pregnancy.Methods A total of 15 patients with gestational hypertension(the gestational hypertension group)and 11 patients with preeclampsia(the preeclampsia group)admitted to this hospital from September 2020 to May 2021 were selected,and 12 normal pregnant women(the control group)were selected during the same period.Flow cytometry was used to detect the expression of CD19^(+)B lymphocytes,CD19^(+)CD24^(hi)CD38^(hi)regulatory B lymphocytes,and IL-10 in peripheral blood of three groups of pregnant women before termination of pregnancy.Results There was no statistically significant difference in the percentage of serum CD19+B lymphocytes among the three groups(P>0.05).The results of pairwise comparison showed that the percentage of serum CD19^(+)CD24^(hi)CD38^(hi)regulatory B lymphocytes in the control group was higher than that in the gestational hypertension group,and it was higher than that in the preeclampsia group.And the differences between the control group and the gestational hypertension group and the preeclampsia group were statistically significant(P<0.05).The results of pairwise comparison showed that the serum level of IL-10 in the control group was higher than that in the gestational hypertension group,and it was higher than that in the preeclampsia group.And the difference between any two groups was statistically significant(P<0.05).Conclusion CD19^(+)CD24^(hi)CD38^(hi)regulatory B lymphocytes and secreted IL-10 are abnormally expressed in women with hypertensive disorder complicating pregnancy,which may be related to the occurrence and development of gestational hypertension.

关 键 词：妊娠期高血压疾病 妊娠高血压 子痫 调节性B淋巴细胞 白细胞介素-10 流式细胞术 

分 类 号：R714.2[医药卫生—妇产科学]