电针百会和印堂对慢性不可预知的应激抑郁模型大鼠内质网应激的影响  

Effects of electroacupuncture at Baihui(GV20)and Yintang(GV29)on endoplasmic reticulum stress in depressive rats caused by chronic unpredictable mild stress

作　　者：郭卓 吴云楚 徐舒亭 GUO Zhuo;WU Yunchu;XU Shuting(College of Integrated Chinese and Western Medicine of Hebei Medical University,Shijiazhuang 050031,China;Shijiazhuang Hospital of Traditional Chinese Medicine,Hebei Province,Shijiazhuang 050011,China)

机构地区：[1]College of Integrated Chinese and Western Medicine of Hebei Medical University,Shijiazhuang 050031,China [2]Shijiazhuang Hospital of Traditional Chinese Medicine,Hebei Province,Shijiazhuang 050011,China

出　　处：《Journal of Acupuncture and Tuina Science》2023年第6期437-446,共10页针灸推拿医学（英文版）

基　　金：河北省中医药管理局科研计划项目,No.2023130;河北医科大学青年科技创新人才托举培育计划项目,No.PJZR202010.

摘　　要：目的:探讨电针(EA)对慢性不可预知的应激抑郁模型(CUMS)大鼠的疗效及其对内质网应激(ERS)相关蛋白的影响。方法:将32只Sprague-Dawley大鼠随机分为对照组、CUMS组、艾司西酞普兰(ESC)组和EA组。除对照组外,其余组别大鼠均采用慢性不可预知的应激建立抑郁模型。从造模第一天开始,电针组每日对百会穴、印堂穴施以电针治疗,15 min/次;艾司西酞普兰组采用灌胃干预[ESC每日10 mg/(kg·bw)],均干预28 d。实验前和第28天通过称量体质量、糖水偏好实验以及旷场实验观察各组大鼠的体质量增长情况、糖水偏好指数、旷场实验总移动距离以及中心区停留时间。采用免疫印迹法的免染总蛋白归一法检测大鼠海马和前额叶皮层葡萄糖调节蛋白78(GRP78)、门冬氨酸特异性半胱氨酸蛋白酶12(caspase-12)和CCAAT增强子结合蛋白同源蛋白(CHOP)蛋白的表达水平。同时,采用原位末端凋亡法检测前额叶皮层的细胞凋亡率。结果:电针百会穴和印堂穴后,大鼠的体质量增长量、糖水偏好指数、旷场实验移动距离和中心区停留时间显著改善(P<0.05或P<0.01);与CUMS组比较,大鼠海马中的GRP78、CHOP和前额叶皮质中的caspase-12及CHOP表达显著降低(P<0.05),前额叶皮层的细胞凋亡率显著下降(P<0.05)。结论:电针百会和印堂可显著改善CUMS抑郁模型大鼠的行为学缺陷。其机制可能与调节ERS,抑制细胞凋亡有关。Objective:To explore the effects of electroacupuncture(EA)on rats with chronic unpredictable mild stress(CUMS)-induced depression and proteins related to endoplasmic reticulum stress(ERS).Methods:Thirty-two Sprague-Dawley rats were randomly divided into a control group,a CUMS group,an escitalopram(ESC)group,and an EA group.Except for the control group,rats in other groups were exposed to CUMS.From the 1st day of modeling,the EA group was treated with EA at Baihui(GV20)and Yintang(GV29)every day,15 min each time;the ESC group was given intragastric intervention[ESC 10 mg/(kg·bw)every day],both for 28 d.Body mass measurement,open field test(OFT),and sucrose preference test(SPT)in each group were performed before(day 0)and after experiment(day 28)to record body mass growth,sugar preference index,and total distance and duration of central area in OFT.The protein expression levels of glucose-regulated protein 78(GRP78),caspase-12,and C/EBP homologous protein(CHOP)in the hippocampus and frontal cortex were detected by stain-free total protein normalization method of Western blotting.Meanwhile,the TdT-mediated dUTP-biotin nick end labeling assay was performed to determine the percentage of cell death in the frontal cortex.Results:After EA at Baihui(GV20)and Yintang(GV29),the body mass growth,sugar preference index,and total distance and duration of central area in OFT were effectively improved(P<0.05 or P<0.01);compared with the CUMS group,the expression levels of GRP78 and CHOP in the hippocampus,and caspase-12 and CHOP in the prefrontal cortex of rats were significantly reduced(P<0.05),and the apoptosis rate of cells in the prefrontal cortex decreased significantly(P<0.05).Conclusion:EA at Baihui(GV20)and Yintang(GV29)can significantly mitigate behavioral deficits of rats with CUMS-induced depression.The mechanism may be related to adjusting the ERS and inhibiting cell apoptosis.

关 键 词：针刺疗法 电针 穴 百会 穴 印堂 抑郁 内质网应激 细胞凋亡 大鼠 

分 类 号：R2-03[医药卫生—中医学]