基于微流控技术的单细胞电学特性研究  

Electrical Characteristics of Single Cells with Microfluidic

作　　者：郑磊 黄明宇[1] 张华丽[1] ZHENG Lei;HUANG Ming-yu;ZHANG Hua-li(School of Mechanical Engineering,Nantong University,Jiangsu Nantong 226019,China)

机构地区：[1]南通大学机械工程学院,江苏南通226019

出　　处：《机械设计与制造》2023年第12期116-118,123,共4页Machinery Design & Manufacture

基　　金：江苏省自然科学基金资助项目(BK20150406);江苏省高校优势学科建设工程资助项目(苏财政2018-192号)。

摘　　要：将微流控技术与库尔特计数器相结合,基于细胞电学特性对细胞进行无标记检测。该检测技术在细胞定量分析及分选中具有很大的优势。这里采用数值仿真的方法研究了成熟红细胞在直流和交流电源下的电学特性,仿真结果表明,不同细胞大小在电极检测区域引起的电信号变化,显示出不同的电学特性。为验证仿真结果,基于微流控技术对浓度为10^(5)个/ml的聚苯乙烯微球,在3V直流电源下所引起的电流变化进行研究。研究结果表明,该模型可以较好的反应细胞在电极检测区域内的电学特性。The microfluidic technology is combined with the Coulter counter to perform label-free detection of cells based on the electrical characteristics of cells.This detection technology has great advantages in cell quantitative analysis and sorting.It uses numerical simulation to study the electrical characteristics of mature red blood cells under DC and AC power supplies.The simulation results show that the electrical signal changes caused by different cell sizes in the electrode detection area show different electrical characteristics.In order to verify the simulation results,based on the microfluidic technology,the current change caused by the polystyrene microspheres with a concentration of 10^(5)/ml under a 3V DC power supply was studied.The research results show that the model can better reflect the electrical characteristics of cells in the electrode detection area.

关 键 词：微流控技术 库尔特计数器 单细胞模型 电学特性 数值仿真 

分 类 号：TH16[机械工程—机械制造及自动化] Q631[生物学—生物物理学] TN24[电子电信—物理电子学] O43[机械工程—光学工程]