川陕花椒根皮提取物对人角质形成细胞辐射损伤的防护作用  

Protective effects of extract from the root bark of Zanthoxylum piasezkii on radiation injury of human keratinocytes

作　　者：王成芳 齐雪松[1] 邵帅[1] 杜树山 苟巧[1] WANG Chengfang;QI Xuesong;SHAO Shuai;DU Shushan;GOU Qiao(National Institute for Radiological Protection,Chinese Centre for Disease Control and Prevention,Key Laboratory of Radiological Protection and Nuclear Emergency,China CDC,Beijing 100088,China;Beijing Normal University,Faculty of Geographical Science,Beijing 100875)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088 [2]北京师范大学地理科学学部,北京100875

出　　处：《辐射防护》2023年第6期657-664,共8页Radiation Protection

摘　　要：放射性皮肤损伤是接受放射治疗的患者常见的并发症,其发病机制复杂,患者个体化差异较大,临床上防治药物疗效良莠不齐,因而积极开发防治放射性皮肤损伤的药物仍十分必要。花椒是中医临床上用于治疗皮肤疾病的常用药,本文研究川陕花椒根皮提取物(ZPE)对人皮肤角质形成细胞HaCaT的辐射防护作用,并初步探讨其机制。研究结果显示,2 mg/mL ZPE对8.0 Gyγ射线照射引起的HaCaT细胞损伤具有防护作用,可减少细胞凋亡和坏死,其作用机制可能与降低细胞炎症因子IL-1β和IL-6的分泌、细胞内ROS水平以及p38和JNK蛋白磷酸化水平有关。本文的研究可为放射性皮肤损伤防治药物的研发提供理论依据。Radiation-induced skin injury is a common complication of patients receiving radiotherapy.Its pathogenesis is complicated and varies greatly with individual patients.Therefore,it is necessary to develop the drugs for prevention and treatment of radiation-induced skin injury.Chinese prickly ash(Zanthoxylum bungeanum)is a common medicine used for skin diseases in traditional Chinese medicine.In this study,the radio-protective effect of the root bark extract of Zanthoxylum piasezkii(ZPE)on HaCaT was investigated and its mechanism was preliminary discussed.The results showed that 2 mg/mL ZPE could protect HaCaT cells from damage induced by 8.0 Gyγray irradiation,and could reduce apoptosis and necrosis of HaCaT cells.The mechanism may be related to the reduction of the secretion of IL-1βand IL-6,the level of ROS and the phosphorylation of p38 and JNK proteins.This work provides a theoretical basis for the development of preventive and therapeutic drugs for radiation-induced skin injury.

关 键 词：放射性皮肤损伤 川陕花椒 辐射防护 人角质形成细胞 

分 类 号：R818[医药卫生—放射医学]