小西葫芦黄花叶病毒甜瓜分离物的基因组及其侵染性克隆  

The genome and infectious clone of zucchini yellow mosaic virus melon isolate

作　　者：刘莉铭[1,2] 彭斌 康保珊[1,2] 吴会杰[1,2] 刘茜 古勤生[1,2] LIU Liming;PENG Bin;KANG Baoshan;WU Huijie;LIU Xi;GU Qinsheng(Henan Key Laboratory of Fruit and Cucurbit Biology/Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China;Zhongyuan Research Center,Chinese Academy of Agricultural Sciences,Xinxiang 453500,Henan,China)

机构地区：[1]河南省果树瓜类生物学重点实验室·中国农业科学院郑州果树研究所,郑州450009 [2]中国农业科学院中原研究中心,河南新乡453500

出　　处：《中国瓜菜》2023年第12期9-15,32,共8页China Cucurbits And Vegetables

基　　金：河南省科技攻关项目(202102110075);国家现代农业产业技术体系(CARS-25-2023-G20);中国农业科学院科技创新工程(CAAS-ASTIP-2022-ZFRI-09)。

摘　　要：病毒侵染性克隆是病毒与寄主互作研究的有力工具,分析了小西葫芦黄花叶病毒(zucchini yellow mosaic virus,ZYMV)甜瓜分离物CH-87的基因组序列和分子变异,构建了具有侵染性的全长cDNA克隆。结果显示,CH-87分离物基因组全长为9592 nt,与其他分离物的全基因组核苷酸和多聚蛋白氨基酸的序列一致性平均值分别为91.42%、96.45%。基于CP蛋白和多聚蛋白氨基酸序列的系统进化分析显示,CH-87分离物与美国的BL-67南瓜分离物亲缘关系最近,与中国的CN∶Lc∶17丝瓜分离物亲缘关系次之,均聚于亚组Ⅴ中。接种试验显示,CH-87分离物的克隆具有侵染性,能系统侵染甜瓜、黄瓜、西瓜和西葫芦,经接种产生的病毒后代也具有侵染性。Viral infectious clone is a powerful tool for studying the interaction between virus and host.In this study,the genome sequence of zucchini yellow mosaic virus(ZYMV)melon isolate CH-87 was cloned and analyzed,and its full-length cDNA clone was constructed.The results showed that the genome of isolate CH-87 was 9592 nt in length,and the average nucleotide and amino acid sequence identities between CH-87 and other isolates were 91.42%and 96.45%,respectivly.Phylogenetic analysis based on CP and polyprotein amino acid sequences showed that CH-87 was most closely related to isolate BL-67 from The United States,followed by isolate CN∶Lc∶17 from China,and they all clustered in subgroupⅤ.The inoculation showed that the infectious clone was successfully constructed,and it could systematically infect melon,cucumber,watermelon and zucchini.The progeny produced from the clone was infectious by mechanical inoculation.

关 键 词：小西葫芦黄花叶病毒 甜瓜分离物 基因组 侵染性克隆 

分 类 号：S652[农业科学—果树学]