利用InDel标记鉴定汴早九号大白菜杂交种纯度  

Hybrid purity identification of Chinese cabbage Bianzao No.9 by InDel marker

作　　者：冯健起 王培云 蔡亚平 丁聪 王惠云 生园园 FENG Jianqi;WANG Peiyun;CAI Yaping;DING Cong;WANG Huiyun;SHENG Yuanyuan(Kaifeng Academy of Agriculture and Forestry,Kaifeng 475004,Henan,China;Kaifeng Seed Industry Development Center,Kaifeng 475004,Henan,China;Kaifeng Agricultural and Rural Development Service Center,Kaifeng 475004,Henan,China)

机构地区：[1]河南省开封市农林科学研究院,河南开封475004 [2]河南省开封市种业发展中心,河南开封475004 [3]河南省开封市农业农村发展服务中心,河南开封475004

出　　处：《中国瓜菜》2023年第12期33-38,共6页China Cucurbits And Vegetables

基　　金：河南省重点研发专项(221111110100)。

摘　　要：为了准确高效地鉴定大白菜汴早九号杂交种的纯度,从已公布的基于大白菜全基因组开发的32对InDel核心引物中筛选获得了InDel引物BrID90029,在汴早九号父母本间呈互补带型且易辨别。利用该InDel引物对288份汴早九号杂交种F1进行纯度鉴定,与田间形态的鉴定结果吻合度高达99.29%,表明InDel引物BrID90029可用于大白菜汴早九号杂交种的纯度鉴定。该结果为规模化检测大白菜杂交种汴早九号的纯度提供了技术依据,具有重要的应用价值。To identify seed purity of Chinese cabbage(Brassica rapa)Bianzao No.9 accurately and efficiently,32 pairs of published InDel core primers based on the whole genome of Chinese cabbage were used.The InDel primer BrID90029 was selected to identify the seeds purity of Bianzao No.9.The bands showed diversity between parents and were complementary with high clarity.Based on the primer,288 Chinese cabbage F1 hybrids Bianzao No.9 were identified for purity.Molecular detection matched field phenotypic identification with 99.29%consistency.Therefore,the InDel primer BrID90029 can be applied to accurately and efficiently identify the purity of Chinese cabbage hybrid Bianzao No.9.This study provides a technical basis for large-scale detection of the purity of Bianzao No.9,and has a significant application value.

关 键 词：大白菜 汴早九号 杂交种 纯度鉴定 

分 类 号：S634.1[农业科学—蔬菜学]