韭菜灰霉病菌对嘧霉胺和咯菌腈抗性评价及抗性菌株适合度分析  

Evaluation of resistance of Chinese chive gray mold(Botrytis cinerea)against pyrimethanil and fludioxonil and the suitability of resistant strains

作　　者：张艳丽 陈娟[2] 张艺潇 陈燕玲 ZHANG Yanli;CHEN Juan;ZHANG Yixiao;CHEN Yanling(Pingliang Agricultural Products Quality Safety and Inspection and Testing Center,Pingliang 744000,Gansu,China;Gansu Academy of Agricultural Sciences,Lanzhou 730000,Gansu,China)

机构地区：[1]平凉市农产品质量安全与检验检测中心,甘肃平凉744000 [2]甘肃省农业科学院,兰州730000

出　　处：《中国瓜菜》2023年第12期107-112,共6页China Cucurbits And Vegetables

基　　金：平凉市创新基地专项(018-1-05/1-7)。

摘　　要：为研究韭菜灰霉病菌对嘧霉胺和咯菌腈的抗性水平及抗性菌株适合度的影响,在甘肃不同区域采集并分离出54株韭菜灰霉病菌,采用抗药性MIC法(最低抑制浓度法)、菌丝生长速率法分别测定嘧霉胺和咯菌腈对灰霉病菌的最低抑制浓度及抗性菌株的敏感性,以此划分敏感菌株和抗性菌株,确定其抗性水平,分析抗性菌株的遗传稳定性及适合度。结果表明,嘧霉胺和咯菌腈对54株灰霉病病菌的最低抑制浓度分别为4.80和0.15μg·mL^(-1),抗性频率分别为35.19%和9.26%。灰霉病菌对嘧霉胺和咯菌腈的抗性分别表现为中抗、高抗和低抗水平;低抗菌株抗嘧霉胺特性不能稳定遗传,转代培养后有向敏感菌株发展的趋势。对嘧霉胺产生抗性的菌株,其适合度显著低于敏感菌株,在与敏感菌株的竞争中不占优势;对咯菌腈产生抗性的低抗菌株,其适合度与敏感菌株相当。In order to clarify the sensitivity level of the pathogen of Chinese chiv gray mold to pyrimethanil and fludioxonil in different regions of Gansu province,fifty-four strains were collected and isolated from the disease plants in different regions.In order to divide the sensitive and resistant strains,the minimum inhibitory concentrations of pyrimethanil and fludioxonil were determined by the MIC method(minimum inhibitory concentration method).The susceptibility of resistant strains to pyrimethanil and fluxonil was determined by mycelium growth rate method,and their resistance levels to the two fungicides were also tested.The genetic stability and fitness of resistant strains were further analyzed.The minimum inhibitory concentrations of pyrimethanil and fludioxonil against fifty-four strains of Botrytis cinerea were 4.8,0.15μg·L^(-1),respectively.The resistance frequency was 35.19%and 9.26%respectively.The resistance of the pathogen of B.cinerea to pyrimethanil was medium and high,low resistance to fludioxonil.The low resistance strains against pyrimethanil can not be inherited stably.However,there was a trend towards greater sensitivity to pyrimethanil after subculture.The suitability of pyrimetrium-resistant strains was significantly lower than that of pyrimetrium sensitive strains.The pyrimetrium-resistant strains were not superior in competition with sensitive strains.The suitability of the fluroxonitrile resistant strains was similar to that of the fluroxonitrile sensitive strains.

关 键 词：韭菜 灰霉病 嘧霉胺 咯菌腈 抗性评价 适合度 

分 类 号：S633.3[农业科学—蔬菜学]