腐殖酸营养液对镉胁迫下树仔菜幼苗生理生化特性及重金属积累的影响  

Effects of humic acid nutrient solution on physio-biochemistry characteristic and heavy metal accumulation of Sauropus androgynus seedlings under cadmium stress

作　　者：范琼[1] 酒元达 叶海辉[1] 冯剑 邹冬梅[1] 苏初连 赵敏[1] FAN Qiong;JIU Yuanda;YE Haihui;FENG Jian;ZOU Dongmei;SU Chulian;ZHAO Min(Analysis and Testing Center,Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables/Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable,Ministry of Agricultural and Rural Affairs,Haikou 571101,Hainan,China)

机构地区：[1]中国热带农业科学院分析测试中心·海南省热带果蔬产品质量安全重点实验室·农业农村部亚热带果品蔬菜质量安全控制重点实验室,海口571101

出　　处：《中国瓜菜》2023年第12期120-128,共9页China Cucurbits And Vegetables

基　　金：海南省自然科学基金(320QN301);海南省热带果蔬产品质量安全重点实验室开放课题(KFKT2021001)。

摘　　要：为探讨镉(Cd)胁迫下腐殖酸营养液对树仔菜幼苗生理生化特性及重金属积累的影响,对不同Cd浓度(0、15、25、45 mg·kg^(-1))胁迫下施用腐殖酸营养液(AF)和不施用腐殖酸营养液(CK)的树仔菜幼苗进行盆栽试验。结果表明,与CK相比,AF处理可有效提高树仔菜总生物量、叶面积和叶片数。在Cd浓度为45 mg·kg^(-1)时,AF处理促进了树仔菜叶片和根部对Cd的吸收以及叶片对Zn的富集,分别显著增加了134.7%、39.0%和36.9%,叶片和根部MDA含量分别显著增加了7.0%和19.6%。AF处理能有效提升抗氧化酶活性,在没有Cd胁迫时,AF处理的树仔菜根部和叶片POD活性、叶片SOD活性分别显著高于CK 187.6%、35.1%和20.6%,且随着Cd浓度增加,AF处理根部的CAT和SOD活性均呈升高趋势。综上,腐殖酸营养液促进了高Cd胁迫下树仔菜对Cd的吸收,但是树仔菜生长发育情况却没有受到影响,主要是由于抗氧化酶保护系统缓解了脂膜过氧化伤害,增强了树仔菜的耐Cd能力。In order to investigate the effects of humic acid nutrient solution on physio-biochemistry and heavy metal content of Sauropus androgynus seedlings under cadmium(Cd)stress,pot experiments with humic acid nutrient solution(AF)treatment and without humic acid nutrient solution(CK)treatment were conducted under different soil Cd concentrations(0,15,25,and 45 mg·kg^(-1)).The results showed that the application of AF can effectively improve the total biomass,leaf area and leaf number of Sauropus androgynus.At Cd45 the AF application promoted the accumulation of Cd in leaves and roots and Zn in leaves of S.androgynus,which increased by 134.7%,39.0%and 36.9%,respectively.MDA content in leaves and roots increased by 7.0%and 19.6%,respectively.The AF treatment can effectively increase the activity of antioxidant enzymes.Under no Cd,the POD enzyme activity in roots and leaves and SOD enzyme activity in leaves of S.androgynus treated with AF application were 187.6%,35.1%and 20.6%higher than those treated with CK,respectively.And the CAT and SOD enzyme activity in roots increased with the concentration of Cd.Therefore,AF promoted the Cd accumulation of S.androgynus under high Cd stress,but the growth of S.androgynus were not affected,mainly because the protection system of antioxidant enzymes mitigated the damage of lipid membrane peroxidation and increased the Cd tolerance of S.androgynus.

关 键 词：树仔菜 腐殖酸营养液 CD胁迫 生理生化特性 

分 类 号：S649[农业科学—蔬菜学]