基于高通量测序的玉米和刺梨种植地土壤细菌群落多样性  

Microbial Community Diversity in Zea mays and Rosa roxburghii Planting Soil Based on High-throughput Sequencing

作　　者：张龙艳 陈京晶 刘兴 冯沙沙 彭美鸾 赵强 文达 ZHANG Longyan;CHEN Jingjing;LIU Xing;FENG Shasha;PENG Meiluan;ZHAO Qiang;WEN Da(School of Geography and Resources,Guizhou Education University,Guiyang,Guizhou 550018,China)

机构地区：[1]贵州师范学院地理与资源学院,贵州贵阳550018

出　　处：《贵州农业科学》2023年第12期23-30,共8页Guizhou Agricultural Sciences

基　　金：贵州省科技计划项目[黔科合基础-ZK(2022)一般328];贵州省教育厅青年科技人才成长项目[黔教合KY字(2022)300];贵州师范学院博士基金项目(2021BS017);贵州师范学院大学生创新创业训练计划项目(S202114223029)。

摘　　要：【目的】探明玉米和刺梨土壤细菌群落分类和功能多样性,为种植业结构调整中采用刺梨替代玉米提供理论依据。【方法】利用16S rDNA高通量测序手段,对玉米和刺梨种植地土壤进行分析,了解不同种植地类型下细菌群落分类和功能多样性的异同。【结果】不同种植地土壤细菌物种分类组成存在差异。链霉菌属(Streptomyces)和MND1样地间差异显著,且均是刺梨地土壤中的丰度更高。相比玉米地细菌群落结构的复杂性,刺梨地细菌群落构结点和边越少,复杂性越低,网络抗毁性越强,恢复力稳定性越好,更有利于抵抗微环境的变化。与信号传导和细胞过程相关的孔蛋白在玉米地的丰度显著高于刺梨地,异戊烯基转移酶则存在相反的情况。【结论】玉米和刺梨地土壤细菌群落构成存在差异,刺梨地土壤中链霉菌属(Streptomyces)和MND1属含量均较高;刺梨地土壤细菌群落构成较简单,具有更强的环境变化抵抗能力;与跨膜运输相关的孔蛋白在玉米地土壤细菌中丰度更高。【Objective】The taxonomy and functional diversity of soil microbial community in Zea mays and Rosa roxburghii were studied to provide the theoretical basis for R.roxburghii replacing Z.mays in planting structure adjustment.【Method】16S rDNA high-throughput sequencing was used to analyze the soil of corn and R.roxburghii and to understand the differences and similarities of bacterial community taxonomy and functional diversity under different planting soil types.【Result】There were differences in bacteria taxonomic composition of different planting soils.Streptomyces and MND1 varied significantly among different sample plots,and the abundance in R.roxburghii soil was higher.Compared to the complexity of bacterial community in Z.mays soil,the fewer nodes and edges of bacterial community,the lower the complexity in R.roxburghii soil,the stronger the network invulnerability,the better the stability of recovery ability,which was more beneficial to resist the micro-environment changes.The abundance of porin proteins related to signal transduction and cellular process was higher significantly in Z.mays soil than those in R.roxburghii soil,while the opposite was true for prenyltransferase.【Conclusion】There were differences in bacterial community composition between Z.a mays soil and R.roxburghi i soil;Streptomycetes and MND1 content were higher in R.roxburghii soil;The bacterial community composition in R.roxburghii soil was simpler,and more resistant to environmental changes.The abundance of porin proteins related to transmembrane transport was higher in Z.mays soil.

关 键 词：土壤细菌 高通量测序 细菌群落 功能多样性 耕作土壤 

分 类 号：S154.3[农业科学—土壤学]