镉污染对山区水库消落带湿地土壤细菌群落结构及多样性的影响  

Effects of cadmium pollution on soil bacterial structure and diversity in riparian wetland of mountain reservoir

作　　者：陈海生[1] 姜伟 徐蒋来 刘守平[2] 罗文[1] 高永胜[1] 蔡林生 方昉[1] 姚金兰[1] CHEN Hai-sheng;JIANG Wei;XU Jiang-lai;LIU Shou-ping;LUO Wen;GAO Yong-sheng;CAI Lin-sheng;FANG Fang;YAO Jin-lan(Zhejiang Tongji Vocational College of Science and Technology,Hangzhou 311231,China;Taizhou Academy of Agricultural Sciences in Zhejiang Province,Linhai,Zhejiang 317000,China)

机构地区：[1]浙江同济科技职业学院,杭州311231 [2]浙江省台州市农业科学研究院,浙江临海317000

出　　处：《西南农业学报》2023年第10期2273-2280,共8页Southwest China Journal of Agricultural Sciences

基　　金：浙江省科技厅项目(LGN18C030002);浙江省水利科技计划项目(RC1826、RC1939);农业部第三次全国农作物种质资源普查与收集行动(111821301354052030)。

摘　　要：【目的】研究镉(Cd)污染对浙江省山区水库消落带湿地土壤细菌群落结构及多样性的影响,探求有利于Cd污染湿地土壤修复的耐Cd细菌菌落。【方法】采用Illumina Miseq高通量测序技术,分析Cd污染对消落带湿地建群种植物落羽杉根际土壤细菌群落结构和多样性指数的影响。Cd处理浓度分别为0、100和400 mg/kg,并分别标记为Cd0、Cd100和Cd400。【结果】研究区所有土壤样品共有34门、96纲、202目、313科、502属土壤细菌。低浓度Cd污染时,消落带湿地植物落羽杉根际土壤细菌Chao1指数和Shannon指数与对照相比大幅升高,而高浓度Cd污染时,其Chao1指数和Shannon指数大幅下降。各处理特有的细菌OTUs也呈同样的趋势。不同程度Cd污染条件下,消落带湿地落羽杉根际土壤细菌群落结构在门和属水平上发生明显变化。随着Cd污染程度的加剧,消落带湿地土壤放线菌门和浮霉菌门相对丰度呈持续下降趋势,拟杆菌门和芽单胞菌门相对丰度呈持续升高趋势。土壤变形菌门和髌骨菌门相对丰度呈先下降后上升趋势,而土壤酸杆菌门、棒状杆菌门、疣微菌门、硝化螺旋菌门和厚壁菌门相对丰度呈先上升后下降趋势。变形菌门、拟杆菌门、髌骨菌门、芽单胞菌门在高浓度Cd污染下为湿地土壤细菌群落的主体。土壤罗思河小杆菌属相对丰度在低浓度Cd污染时呈下降趋势,而在高浓度Cd污染时呈升高趋势,土壤鞘氨醇单胞菌属的相对丰度在低浓度Cd污染时呈上升趋势,在高浓度Cd污染时呈下降趋势。土壤假节杆菌属、黏液杆菌属以及红假单胞菌属的相对丰度随着Cd浓度的增加一直呈下降趋势。土壤黄杆菌属的相对丰度随着Cd污染程度加剧呈持续升高趋势。【结论】在Cd400处理组中有大量的细菌罗思河小杆菌属、Gemmatimonadaceae-unclassified、马赛菌属、Candidate-Adlerbacteria-unclassified、Chitinophagaceae-unclassifie【Objective】The present paper aimed to study the effects of cadmium pollution on soil bacterial structure and diversity in riparian wetlands of mountain reservoir,and to find out the cadmium-resistant bacteria which were beneficial to the remediation of cadmium pollution.【Method】The high throughput sequencing method of Illumina Miseq was used to analyze the effects of cadmium stress on bacterial community and diversity in rhizosphere soils of Taxodium distichum planting in riparian wetlands of Changtan reservoir,Zhejiang province.The concentrations of cadmium were set at 0,100,400 mg/kg,respectively,and were marked as Cd0,Cd100,Cd400,correspondingly.【Result】The cadmium stress could cause changes in both bacterial diversity and community structure.There were 34 phyla,96 classes,202 orders,313 families,and 502 genera in the rhizosphere soil of T.distichum planting in riparian wetlands of Changtan reservoir under different gradients of cadmium pollution.Low cadmium stress might raise the Chao1 index and Shannon index of soil bacterial community and high cadmium stress might reduce the Chao1 index and Shannon index of soil bacterial community.With the increase of Cd stress,the relative abundance of Actinobacteri and Planctornyces in the soil of riparian wetland showed a downward trend all the time,while the relative abundance of Bacteroidetes and Gemmatimonadetes were on the increase all the time.The relative abundance of soil Proteobacteria and Patescibacteria decreased first and then increased.The relative abundances of Acidobacteria,Rokubacteria,Errucomicrobia,Nitrospirae and Firmicutes in soil increased first and then decreased.Proteobacteria,Bacteroidetes,Patescibacteria and Gemmatimonadetes were the dominant groups in high Cd concentration stress.With the increase of Cd stress,the relative abundance of Pseudarthrobacter,Muciaginibacter and Rhodopseudomonas in the soil of riparian wetland showed a downward trend all the time,while the relative abundance of Flavobacterium was on the increase all the ti

关 键 词：镉 消落带湿地 细菌群落结构 高通量测序 落羽杉 

分 类 号：S154[农业科学—土壤学]