基于代谢组学探讨犊牛失明原因  

Discussion on cause of calf blindness based on metabonomics

作　　者：王建东 唐玉林 郭亚男 高海慧 侯鹏霞 于洋 郭延生[2] WANG Jian-dong;TANG Yu-lin;GUO Ya-nan;GAO Hai-hui;HOU Peng-xia;YU Yang;GUO Yan-sheng(Institute of Animal Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China;College of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏农林科学院动物科学研究所,银川750002 [2]宁夏大学农学院,银川750021

出　　处：《西南农业学报》2023年第10期2281-2291,共11页Southwest China Journal of Agricultural Sciences

基　　金：农业高质量发展和生态保护科技创新示范课题(NGSB-2021-12-03);宁夏规模化奶牛场病毒性腹泻病毒监测及疫病防控技术应用示范项目(2023CJE09028)。

摘　　要：【目的】进一步研究犊牛失明的原因,为降低犊牛先天失明的发生率,减少养殖行业经济损失提供理论支持。【方法】选择宁夏地区某牛场出生日期接近的失明犊牛及其母亲、正常犊牛,采集血液收集血浆样本,通过代谢组学分析,对失明犊牛及其母亲、正常犊牛之间差异代谢产物及通路变化进行研究。【结果】将OPLS-DA的VIP值>1和单变量统计分析P<0.05作为显著性差异代谢物的标准,失明犊牛与正常犊牛(VaS.vs.VaL)比较组共筛选出256种差异代谢物,其中正离子模式下144种,负离子模式下112种。失明犊牛与其母亲(VaS.vs.CS)比较组共筛选出177种差异代谢物,其中正离子模式下108种,负离子模式下69种。失明犊牛与其母亲相比,视紫红质含量显著降低,log2FC达-2.87。失明犊牛与其母亲相比,P<0.05差异代谢通路共有30个,矫正后P<0.05的差异代谢通路共有10个,其中胆汁分泌代谢通路分布差异代谢物最多,失明犊牛相较于其母亲有4个代谢物显著上调,9个代谢产物显著下调。失明犊牛与正常犊牛相比,P<0.05差异代谢通路共有12个,矫正后P<0.05的差异代谢通路共有16个,其中胆汁分泌代谢通路分布差异代谢物最多,与正常犊牛相比,失明犊牛有5个代谢物显著上调,4个代谢产物显著下调。【结论】失明犊牛与其母亲、正常犊牛相比胆汁分泌代谢通路均产生显著性差异,研究认为犊牛失明可能是机体自身存在肝脏方面的疾病,影响胆汁的正常分泌,导致正常摄入的脂溶性维生素A不能被吸收利用,进而影响犊牛视力。另外,失明犊牛与其母亲相比,在机体维生素A水平没有显著性差异的条件下,影响光转导级联反应的视紫红质含量显著下调,说明编码视紫红质蛋白的视紫红质基因(RHO)突变,视紫红质蛋白缺乏,可能是导致犊牛失明的另一原因。【Objective】The present paper aimed to further study the causes of calf blindness and provide theoretical support for reducing the incidence of congenital blindness of calves and reducing the economic losses of the breeding industry.【Method】A cattle farm was selected in Ningxia,whose birth date was close to that of blind calves and their mothers,and normal calves.Blood and plasma samples were collected.Through metabonomic analysis,the differential metabolites and pathway changes between blind calves and their mothers,and normal calves were studied.【Result】The VIP value of OPLS-DA>1 and the univariate statistical analysis P<0.05 were used as the criteria for significantly different metabolites.A total of 256 different metabolites were screened from the comparison group of blind calves and normal calves(VaS.vs.VaL),including 144 in the positive ion mode and 112 in the negative ion mode.A total of 177 different metabolites were screened from the comparison group between the blind calf and its mother(VaS.vs.CS),including 108 in the positive ion mode and 69 in the negative ion mode.The content of rhodopsin in the blind calf was significantly lower than that of its mother,with log_2FC reaching-2.87.Compared with its mother,there were 30 differential metabolic pathways with P<0.05 in total in blind calves,and 10 differential metabolic pathways with corrected P<0.05 in total.Among them,bile secretion metabolic pathway had the largest distribution of differential metabolites.Compared with its mother,4 metabolites in blind calves were significantly up-regulated,and 9 metabolites were significantly down-regulated.Compared with normal calves,there were 12 differential metabolic pathways with P<0.05 in total in blind calves,and 16 differential metabolic pathways with corrected P<0.05 in total.Among them,bile secretion metabolic pathway distributed the most differential metabolites.Compared with normal calves,there were 5 metabolites significantly up-regulated and 4 metabolites significantly down-regulated in blind

关 键 词：犊牛 失明 代谢组学 视紫红质 维生素A缺乏症 

分 类 号：S857.3[农业科学—临床兽医学]