秦皇岛地区克氏原螯虾源嗜水气单胞菌分离及生物学特征分析  

Isolation and biological characteristics of Aeromonas hydrophila from Procambarus clarkii

作　　者：苏蒙蒙 王璐 赵兴旺 韩业越 张艳英[1] SU Mengmeng;WANG Lu;ZHAO Xingwang;HAN Yeyue;ZHANG Yanying(Hebei Normal University of Science&Technology/Key Laboratory of Preventive Veterinary Medicine in Hebei Province,Qinhuangdao 066004,China;Qianxi County Bureau of Agriculture,Animal Husbandry and Fisheries,Qianxi 064300,China;Qinhuangdao Aquatic Germplasm Resources Protection Zone Management Office,Qinhuangdao 066000,China)

机构地区：[1]河北科技师范学院/河北省预防兽医学重点实验室,河北秦皇岛066600 [2]迁西县农业畜牧水产局,河北迁西064300 [3]秦皇岛市水产种质资源保护区管理处,河北秦皇岛066000

出　　处：《河北渔业》2023年第12期27-32,F0003,共7页

基　　金：中国-阿拉伯合作项目(项目编号:SACF/KF2018004);河北省重点研发计划项目(项目编号:19226628D);河北省教育厅重点项目(项目编号:ZD2019092);河北省第三期现代农业产业技术体系河北省创新团队建设项目海淡水养殖疫病防控及质量控制岗(HBCT2023220207);河北省预防兽医学重点实验室绩效后补助项目(项目编号:20567621H);特色海产品创新团队近海绿色碳汇综合养殖岗(项目编号:HBCT2018170204)。

摘　　要：为鉴定引起克氏原螯虾死亡的病原菌及分析其生物学特征,从秦皇岛地区病死的疑似细菌感染的克氏原螯虾的肝脏和胰腺分离出1株病原菌,暂定名为ProcQHD。经细菌分离鉴定和16S rRNA基因序列分析,该菌株被鉴定为嗜水气单胞菌。通过人工感染动物试验、PCR法及K-B药敏纸片对其致病性、毒力基因、耐药基因及耐药性进行了研究。结果表明,ProcQHD株的LD_(50)为1.0×10^(5) CFU/mL,携带ser、act、exu、lip和Luxs 5种毒力基因;ProcQHD株对替米考星、头孢曲松、环丙沙星、土霉素、诺氟沙星等12种药物高度敏感且携带Tet(A)、Tet(C)、Tet(E)、qepA、gyrB、aac4、aadB、Ant-Ia、Sul-1、LAP-1、SHV 11种耐药基因。分析发现ProcQHD株的某些耐药表型与耐药基因之间有一定相关性。In order to identify the pathogenic bacteria causing the death of Procambarus clarkii and analyze its biological characteristics.A pathogen named ProcQHD was isolated from the liver and pancreas of dead P.clarkii.ProcQHD strain was identified as Aeromonas hydrophila by routine bacterial isolation,identification and 16S rRNA gene sequence analysis.The pathogenicity,virulence gene,drug resistance gene and drug resistance were detected by artificial infection animal experiment,PCR and K-B drug sensitivity test.The results showed that the LD_(50) of ProcQHD strain was 1.0×10^(5) CFU/mL,carried five virulence genes ser,act,exu,lip and LuxS;ProcQHD strain was highly sensitive to 12 kinds of drugs,such as tilmicosin,ceftriaxone,ciprofloxacin,oxytetracycline and norfloxacin.It carried 11 kinds of resistance genes,including Tet(A),Tet(C),Tet(E),qepA,gyrB,aac4,aadB,Ant-Ia,Sul-1,LAP-1,SHV,etc.There was some correlation between drug resistance phenotype and resistance gene.

关 键 词：克氏原螯虾(Procambarus clarkii) 嗜水气单胞菌(Aeromonas hydrophila) 致病性 毒力基因 耐药性 耐药基因 

分 类 号：S968.22[农业科学—水产养殖]