冠心宁治疗急性心肌梗死后心力衰竭大鼠作用机制研究  

Mechanistic action of Guanxinning on heart failure in a rat model of acute myocardial infarction

作　　者：廖骏 沈玮芸 孙启银[1] 柳元化[1] LIAO Jun;SHEN Wei-yun;SUN Qi-yin;LIU Yuan-hua(Department of Cardiovascular Medicine,The First People's Hospital of Huzhou,Huzhou,Zhejiang 313000,China)

机构地区：[1]浙江省湖州市第一人民医院心血管内科,湖州313000

出　　处：《浙江中西医结合杂志》2023年第12期1084-1088,1100,共6页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：江省医学会临床科研基金项目(No.2021ZYC-A136)。

摘　　要：目的观察冠心宁治疗急性心肌梗死后心力衰竭大鼠的作用机制。方法将50只SPF级雄性大鼠通过结扎左冠状动脉前降支的方法建立急性心肌梗死后心力衰竭模型大鼠,造模过程中共有5只大鼠死亡,将造模成功的45只大鼠采用随机数字表法分为空白对照组、氯吡格雷组及冠心宁组,每组各15只。灌胃4周后,观察各组大鼠心肌超微结构变化,检测心肌组织中磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)表达、心肌组织中氧化应激因子[活性氧(ROS)、细胞总抗氧化能力(T-AOC)]、血清肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)、心脏彩超指数[左室室间隔收缩期厚度(IVSs)、左室室间隔舒张期厚度(IVSd)、左室短轴缩短率(LVFS)]。结果与空白对照组比较,氯吡格雷组及冠心宁组大鼠的心肌超微结构变化更显著,且冠心宁组对大鼠心肌超微结构的炎性损伤明显减轻。与空白对照组比较,氯吡格雷组、冠心宁组心肌组织中PI3K和AKT均升高[PI3K:(0.75±0.09)、(0.84±0.11)比(0.59±0.05),AKT:(0.76±0.08)、(0.91±0.13)比(0.71±0.04),P<0.05]。与空白对照组比较,氯吡格雷组、冠心宁组心肌组织中ROS降低[(28.58±2.27)、(21.43±2.18)比(39.71±2.46),P<0.05],T-AOC水平升高[(0.28±0.05)mmol/mg、(0.35±0.09)mmol/mg比(0.13±0.03)mmol/mg,P<0.05]。与空白对照组比较,氯吡格雷组、冠心宁组血清TNF-α水平降低[(78.64±8.55)ng/L、(68.85±6.76)ng/L比(104.85±10.89)ng/L,P<0.05],TGF-β1水平升高[(2176.39±174.39)ng/L、(2314.71±179.58)ng/L比(1652.28±146.93)ng/L,P<0.05]。与空白对照组比较,氯吡格雷组、冠心宁组IVSs水平降低[(3.45±0.37)mm、(2.74±0.31)mm比(4.18±0.45)mm,P<0.05],LVFS水平升高[(40.81±2.61)%、(45.82±2.71)%比(35.71±2.52)%,P<0.05],三组IVSd水平无明显差异(P>0.05)。结论冠心宁可改善急性心肌梗死后心力衰竭大鼠心肌超微结构与心功能,降低氧化应激水平及炎症反应,其作用机制可能与�Objective To investigate the mechanistic action of Guanxinning in treating heart failure in a rat model of acute myocardial infarction.Methods Fifty specific pathogen-free grade male rats were selected to establish a heart failure model after acute myocardial infarction by ligating the anterior descending branch of the left coronary artery.During the modeling process,five rats died and the rest of 45 rats were successfully modeled and then randomly divided into blank control,clopidogrel,and Guanxinning treatment groups(n=15 per group).After 4 weeks of gastric lavage treatments,the ultrastructural changes were assessed in the myocardium of each rat and expression of phosphatidylinositol 3-kinase(PI3K)and protein kinase B(AKT),oxidative stress factors,like the reactive oxygen species(ROS),and total cellular antioxidant capacity(T-AOC)in myocardial tissues and serum tumor necrosis factor-αand seα),transforming growth factor-β1(TGF-β1),acardiac ultrasound index[the left ventricular septal systolic thickness(IVSs),left ventricular septal diastolic thickness(IVSd),and left ventricular short axis shortening rate(LVFS)]were analyzed using the corresponding mythologies.Results Compared with the blank control rats,the myocardial ultrastructure changes in the clopidogrel and Guanxinning-treated rats were more obvious,and the inflammatory damage to the myocardial ultrastructure in the Guanxinning group was significantly reduced.Compared with the blank control group,both clopidogrel and Guanxinning-treated rats showed an increase in PI3K and AKT levels in the myocardial tissues(PI3K,0.75±0.09)and 0.84±0.11 vs.0.59±0.05;AKT,0.76±0.08 and 0.91±0.13 vs.0.71±0.04;P<0.05).Moreover,compared with the blank control,the clopidogrel and Guanxinning groups of rats had a decrease in ROS level in the myocardial tissues(28.58±2.27 and 21.43±2.18 vs.39.71±2.46;P<0.05),but an increase in T-AOC level(0.28±0.05 and 0.35±0.09 vs.0.13±0.03 mmol/mg;P<0.05).Compared with the blank control,the serum TNF level in the clopidogrel and

关 键 词：大鼠 急性心肌梗死 心力衰竭 冠心宁 炎症反应 心功能 

分 类 号：R54[医药卫生—心血管疾病]