用于抗糖化功效评价的细胞模型研究现状及应用前景  

Cell model research status and application prospects for the evaluation of anti-glycation efficacy

作　　者：曹晨灿 谭淇丹 刘蕾 杨小玉 陈海峰 Chencan Cao;Qidan Tan;Lei Liu;Xiaoyu Yang;Haifeng Chen(Beijing Key Laboratory of Plant Resources Research and Development,School of Chemistry and Materials Engineering,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]北京工商大学化学与材料工程学院植物资源研究开发北京市重点实验室,北京100048

出　　处：《日用化学工业（中英文）》2023年第12期1451-1458,共8页China Surfactant Detergent & Cosmetics

摘　　要：综述了糖化反应作用机制、晚期糖化终产物(AGEs)的分类、糖化对皮肤的影响、抗糖化功效评价方法以及国内外用于抗糖化功效评价细胞模型的研究现状。根据各细胞功能不同,总结了角质形成细胞、成纤维细胞、免疫细胞、黑色素细胞和血管内皮细胞5种类型的细胞在抗糖化功效评价细胞模型中的应用,分别针对糖化引起的皮肤屏障受损、胶原流失、色素沉着、氧化应激以及炎症反应等问题。根据建模刺激方式的不同,总结了高浓度葡萄糖、甲基乙二醛/乙二醛、AGEs 3种刺激方式在糖化损伤细胞模型中的应用,分别针对糖化反应作用机制中的早期阶段、中期阶段以及AGEs作用阶段。展望了用于抗糖化功效评价的细胞模型应用前景,以期对抗糖化功效评价细胞模型标准方法的建立提供实验基础以及抗糖化功效原料的筛选提供理论依据。This paper reviews the mechanism of glycation reaction,the classification of advanced glycation end products(AGEs),the effects of glycation on skin,the evaluation methods of antiglycation efficacy and the current research status of the cell models used for the evaluation of antiglycation efficacy at home and abroad.According to the different cell functions,the application of five types of keratinocytes(HaCaT),fibroblasts,immune cells(RAW264.7/THP-1),melanocytes and vascular endothelial cells(HUVECs)in the evaluation cell model of anti-glycation efficacy was summarized.They are aimed at the skin barrier damage,collagen loss,pigmentation,oxidative stress and inflammation caused by glycation.According to the different modeling stimulation modes,the application of three stimulation modes of high glucose,methylglyoxal/glyoxal(MGO/GO)and AGEs in the cell model of glycation injury was summarized,aiming at the early stage,middle and late stage of the mechanism of glycation reaction and the action stage of AGEs.This review looks forward to the application prospect of cell models for anti-glycation efficacy evaluation,in order to provide an experimental basis for the establishment of standard methods for anti-glycation efficacy evaluation cell models and provide a theoretical basis for the screening of anti-glycation efficacy raw materials.

关 键 词：抗糖化 细胞模型 化妆品功效评价 晚期糖化终产物(AGEs) 

分 类 号：TQ658[化学工程—精细化工]