一种耐盐复合菌剂的制备和促生作用研究  

Preparation of Compound Halotolerant Bioinoculant and Study on Its Growth-promoting Effect

作　　者：车永梅[1] 刘广超 郭艳苹 叶青[1] 赵方贵[1] 刘新[1] CHE Yong-mei;LIU Guang-chao;GUO Yan-ping;YE Qing;ZHAO Fang-gui;LIU Xin(Key Lab of Plant Biotechnology in Universities of Shandong Province,Life Science College,Qingdao Agricultural University,Qingdao 266109)

机构地区：[1]青岛农业大学生命科学学院山东省高校植物生物技术重点实验室,青岛266109

出　　处：《生物技术通报》2023年第11期217-225,共9页Biotechnology Bulletin

基　　金：国家自然基金委联合项目(U2106229);山东省现代农业产业技术体系(SDAIT-25-05);山东省良种工程项目(2020LZGC008)。

摘　　要：土壤盐渍化是影响作物生长发育和产量的主要环境因素,利用土壤有益微生物治理盐渍化土壤是盐碱地改良的有效途径。本实验室前期从盐渍化土壤中筛选到两株耐盐菌株氧化微杆菌(Microbacterium oxydans)C8和嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia)B4。菌株C8具有解钾、溶有机磷和无机磷及产生长素的能力,菌株B4具有溶有机磷和产生长素的功能。本文研究了C8和B4混合发酵对其功能的影响,结果表明,C8和B4混合培养后,其解钾、溶磷及产生长素的能力显著高于单一菌株。利用正交试验和响应面优化试验对其混合发酵培养基和培养条件进行优化,C8和B4混合发酵最适培养基配方为:葡萄糖10 g/L,酵母膏10 g/L,NaCl 4.5 g/L;最适培养条件为:pH 7.4,温度28.8℃,转速129 r/min,接种量2%,装瓶量20%,培养时间23 h。以烟草为材料,检测复合菌剂对植株生长的作用,结果显示,盐胁迫下C8和B4复合菌剂处理显著促进植株生长。Soil salinization is a major factor seriously limiting crop growth and yield.Using soil beneficial microorganisms for saline field amendment is the reliable and sustainable approach.Two halotolerant bacteria strains,Microbacterium oxydans C8 and Stenotrophomonas maltophilia B4,were isolated from saline field in our previous study.Strain C8 has the ability to dissolve potassium,organic and inorganic phosphorus as well as produce auxin.Strain B4 is capable of solubilizing organic phosphorus and producing auxin.In this paper,the effect of mixed culture of C8 and B4 on their functions was studied.The results showed that the mixed culture of C8 and B4 demonstrated significantly stronger ability of dissolving potassium,phosphorus and produce auxin than a single strain.Orthogonal experiments and response surface test were used to optimize the fermentation medium and condition of C8 and B4 mixture,the results showed that the optimized fermentation medium was as follows:glucose 10 g/L,yeast extract 10 g/L,sodium chloride 4.5 g/L.The optimized fermentation conditions were:pH 7.4,temperature 28.8℃,rotating speed 129 r/min,inoculation amount 2%,the liquid content 20%and culture time 23 h.Using tobacco as material,the growth-promoting effects of compound bioinoculant of C8 and B4 were studied.The results showed that,the compound bioinoculant significantly prompted plant growth under salt stress.

关 键 词：耐盐促生混合菌剂 发酵优化 烟草 促生作用 

分 类 号：S156.4[农业科学—土壤学] Q939.9[农业科学—农业基础科学]