结球甘蓝PRX基因家族全基因组鉴定与逆境条件下的表达分析  

Genome-wide Identification of the PRX Gene Family in Cabbage(Brassica oleracea L.var.capitata)and Expression Analysis Under Abiotic Stress

作　　者：葛雯冬 王腾辉 马天意 范震宇[1] 王玉书[1] GE Wen-dong;WANG Teng-hui;MA Tian-yi;FAN Zhen-yu;WANG Yu-shu(College of Life Sciences,Agriculture and Forestry,Qiqihar Universuty,Qiqihar 161006)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,齐齐哈尔161006

出　　处：《生物技术通报》2023年第11期252-260,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31401908);黑龙江省自然科学基金项目(C2016056);黑龙江省省属高等学校基本科研业务费科研项目(145209513);黑龙江省普通高等学校青年创新人才培养计划(UNPYSCT-2017155)。

摘　　要：Ⅲ类过氧化物酶(class Ⅲ peroxidases, PRX)在植物生长发育和胁迫响应中发挥重要的作用,本研究利用生物信息学方法对结球甘蓝PRX基因家族进行鉴定,预测其结构和功能,并分析PRX基因在逆境条件下的表达模式,旨在明确甘蓝PRX基因家族的进化关系和功能。结果表明,结球甘蓝基因组中共鉴定出125个BoPRX基因家族成员,不均匀的分布在9条染色体上;编码氨基酸173-488 aa,大部分为亲水蛋白;亚细胞定位预测BoPRX基因大部分定位在叶绿体上。系统进化分析将甘蓝PRX蛋白分为5个亚族,每个亚族的成员都含有相似的外显子/内含子结构和蛋白质保守基序;启动子区域分析发现,BoPRX启动子序列中含有多种与激素和逆境等胁迫响应相关的顺式调控元件;基于转录组数据的热图分析显示,BoPRXs表达在叶绿体中最多。荧光定量PCR分析显示,6个BoPRX基因均受NaCl和PEG诱导上调;在ABA处理下,除BoPRX100外其余基因的表达在大部分时间被抑制,这些基因均受到ABA的差异调控,说明这些BoPRX基因都参与了ABA信号通路。综上所述,本研究揭示了PRX的复杂调控依赖于PRX的类型和信号分子,为进一步分析甘蓝PRX家族关键成员的功能提供了有价值的信息。Class III peroxidases(PRX)play an important role in plant growth and development and stress response.In this study,the class III PRX gene family of cabbage was identified by bioinformatics,its function and structure were predicted,and the expression patterns of PRX gene under different abiotic stresses were analyzed,aiming to clarify the functional and evolutionary relationship of PRX gene family in cabbage genome.The results showed that a total of 125 BoPRX gene family members were identified in the cabbage genome,and were unevenly distributed on 9 chromosomes,the encoded 173-488 amino acids,and most were hydrophilic.Subcellular localization predicted that most BoPRX genes were located in chloroplasts.Phylogenetic analysis showed that the members of PRX family genes in the cabbage were classified into 5 groups,and members of each group had similar exon/intron structures and protein-conserved motifs.Promoter region analysis revealed that the BoPRX promoter sequence contained a variety of cis-regulatory elements related to abiotic stress responses such as hormones and stress.Heat map analysis of transcriptome data demonstrated that BoPRXs expression was the highest in chloroplasts.Fluorescence quantitative PCR analysis showed that 6 BoPRX genes were induced to be up-regulated by NaCl and PEG.Under ABA stress,except for BoPRX100,the expressions of the remaining genes were inhibited at most time,the expressions of the BoPRXs were all differentially regulated by ABA,indicating that they were all involved in the ABA signaling pathway.These findings reveal a complex regulation of PRXs that is dependent on the type of PRX,and the signaling molecule.It provides valuable information for further analysis of the functions of key members of the PRX family of cabbage.

关 键 词：甘蓝 PRX基因 逆境胁迫 表达分析 

分 类 号：S635.1[农业科学—蔬菜学] Q943.2[农业科学—园艺学]