中华猕猴桃GRAS基因家族鉴定及低温胁迫表达分析  

Identification of GRAS Gene Family and Expression Analysis Under Low Temperature Stress in Actinidia chinensis

作　　者：毛可欣 王海荣[1] 安淼[1] 刘腾飞[1] 王世金 李健 李国田[1] MAO Ke-xin;WANG Hai-rong;AN Miao;LIU Teng-fei;WANG Shi-jin;LI Jian;LI Guo-tian(Shandong Institute of Pomology,Tai'an 271000;School of Life Science,Yunnan University,Kunming 650000;Northern Kiwi Fruit(Rizhao)Technology Research Institute,Rizhao 276500)

机构地区：[1]山东省果树研究所,泰安271000 [2]云南大学生命科学学院,昆明650000 [3]北方奇异果(日照)技术研究院,日照276500

出　　处：《生物技术通报》2023年第11期297-307,共11页Biotechnology Bulletin

基　　金：山东省农业科学院创新工程项目(CXGC2023A12,CXGC2023G37);山东省农业良种工程项目(2021LZGC007);山东省农业科学院高层次人才引进项目(CXGC2021B34)。

摘　　要：GRAS基因家族广泛参与植物生长和逆境响应,而低温是制约猕猴桃生产和分布的重要因素之一,鉴定猕猴桃GRAS基因家族,分析其在低温胁迫中的表达情况,为猕猴桃的抗寒研究和品种选育提供理论依据。以中华猕猴桃‘红阳’基因组为参考进行GRAS家族保守结构域比对,通过对鉴定到的家族成员进行系统进化树、蛋白理化性质、基因结构、蛋白三级结构、蛋白质motif、顺式作用元件、共线性、密码子偏好性和基因表达模式等进行分析。结果表明,猕猴桃基因组共存在79个GRAS家族成员,分属于8个亚家族,且各亚家族基因、蛋白结构有所差异。顺式作用原件分析显示该家族基因参与多种植物激素、生长发育以及胁迫响应。密码子偏好性分析发现,该家族密码子第3位碱基更偏好使用嘧啶类碱基(G/T)。鉴定到6个基因可能参与猕猴桃低温胁迫过程,并进行荧光定量PCR验证了该猜测。该研究补充了猕猴桃GRAS基因家族鉴定分析的空白,为猕猴桃抗寒研究奠定分子基础。GRAS gene family is widely involved in plant growth and stress response.Low temperature is one of the important factors that restricts the production and distribution of kiwifruit(Actinidia chinensis).The GRAS gene family of kiwifruit was identified,its expression under low temperature stress was analyzed,which may provide a theoretical basis for the research on cold resistance and variety breeding of kiwifruit.The GRAS gene family conserved domains were compared with the genome of A.chinensis‘Hongyang’reference,and the identified family members were analyzed by phylogenetic tree,protein physical,chemical properties,gene structure,protein tertiary structure,protein motif,cis-acting elements,collinearity,codon usage preference and gene expression mode.The results showed that there was a total of 79 GRAS family genes in kiwifruit genome,belonging to 8 subfamilies,and there were differences in gene and protein structures among each subfamily.Analysis of cis-acting elements showed the genes were involved in multiple plant hormone,growth and development,and stress response.Through codon preference analysis,it was found that the third base of GRAS family preferred to use pyrimidine bases G/T.Six genes were identified that may be involved in the low-temperature stress process in kiwifruit,and this hypothesis was validated by fluorescence quantitative PCR.

关 键 词：猕猴桃 GRAS基因家族 密码子分析 低温胁迫 

分 类 号：S663.4[农业科学—果树学] Q943.2[农业科学—园艺学]