基于VEGF/VEGFR信号通路探讨贝伐珠单抗对肠癌SW480细胞生物学行为的影响及其机制  

Exploration on effect and mechanism of bevacizumab on the biological behaviors of intestinal cancer SW480 cells based on the VEGF/VEGFR signal pathway

作　　者：樊叶[1] 李超[1] 汪志兵[1] FAN Ye;LI Chao;WANG Zhi-bing(Department of Gastroenterology,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,Jiangsu,China)

机构地区：[1]南京医科大学附属南京医院·南京市第一医院消化科,江苏南京210006

出　　处：《川北医学院学报》2023年第12期1598-1602,共5页Journal of North Sichuan Medical College

基　　金：江苏省南京市卫生科技发展专项资金项目(ZKX21042)。

摘　　要：目的:基于血管内皮生长因子(VEGF)/血管内皮生长因子受体(VEGFR)信号通路探讨贝伐珠单抗对肠癌细胞生物学行为的影响及其机制。方法:将人肠癌细胞系SW480细胞作为研究对象,采用不同浓度(0、25、50、100、200μg/mL)的贝伐珠单抗处理SW480细胞。采用CCK8试剂盒检测细胞增殖水平;Transwell迁移侵袭实验检测细胞迁移和侵袭能力;Annexin V-FITC双染法检测细胞凋亡情况;Western Blot法检测细胞内VEGF、VEGFR1和VEGFR2蛋白表达水平。比较不同浓度贝伐珠单抗对肠癌细胞增殖、迁移、侵袭、凋亡及其对肠癌细胞内VEGF/VEGFR信号通路的影响。结果:贝伐珠单抗浓度依赖性地抑制肠癌细胞体外增殖、迁移和侵袭(P<0.05)。贝伐珠单抗浓度依赖性地诱导了肠癌细胞的体外凋亡(P<0.05)。随着贝伐珠单抗浓度的增加,VEGF的表达下调,VEGFR1和VEGFR2的表达上调(P<0.05)。结论:贝伐珠单抗能抑制肠癌细胞增殖、迁移和侵袭,诱导细胞凋亡,其机制可能是通过调控VEGF/VEGFR信号通路。Objective:To explore the effect and mechanism of bevacizumab on the biological behaviors of intestinal cancer cells based on vascular endothelial growth factor(VEGF)/vascular endothelial growth factor receptor(VEGFR)signaling pathway.Methods:The human intestinal cancer cell line SW480 cells were selected as the subjects of this study.The SW480 cells were treated with bevacizumab at different concentrations(0,25,50,100,200μg/mL),and Cell Counting Kit-8(CCK8)was used to detect the cell proliferation level,and Transwell migration and invasion test was applied to detect the cell migration and invasion abilities.Fluorescein isothiocyanate(Annexin V-FITC)double staining method was adopted to measure the cell apoptosis,and Western Blot was for the detection of protein expression levels of intracellular VEGF,VEGFR1 and VEGFR2.The effect of different concentrations of bevacizumab on proliferation,migration,invasion,apoptosis and VEGF/VEGFR signaling pathway in colorectal cancer cells was compared.Results:Bevacizumab inhibited the in vitro proliferation,migration and invasion of intestinal cancer cells in a concentration-dependent manner(P<0.05).Bevacizumab induced the in vitro apoptosis of intestinal cancer cells in a concentration-dependent manner(P<0.05).As the concentration of bevacizumab increased,the expression of VEGF was down-regulated while the expressions of VEGFR1 and VEGFR2 were up-regulated(P<0.05).Conclusion:Bevacizumab can inhibit the proliferation,migration and invasion and induce the cell apoptosis of intestinal cancer cells in vitro by regulating the VEGF/VEGFR signaling pathway.

关 键 词：血管内皮生长因子 血管内皮生长因子受体 贝伐珠单抗 肠癌 细胞生物学行为 

分 类 号：R965.1[医药卫生—药理学]