硫酸盐还原菌浓度检测和腐蚀活性监测方法研究进展  

Research progress on methods of concentration detection and corrosive activity monitoring of sulfate reducing bacteria

作　　者：王彦然 唐永帆[1] 肖杰[1] WANG Yanran;TANG Yongfan;XIAO Jie(Research Institute of Natural Gas Technology,PetroChina Southwest Oil&Gasfield Company,Chengdu 610213,China)

机构地区：[1]中国石油西南油气田分公司天然气研究院,四川成都610213

出　　处：《工业水处理》2023年第12期58-65,共8页Industrial Water Treatment

基　　金：西南油气田分公司科研项目(20210305-01)。

摘　　要：国内外已形成多种硫酸盐还原菌(SRB)浓度检测和腐蚀活性监测方法,适用条件各有差异。详细论述了SRB浓度检测和腐蚀活性监测方法的研究进展。首先基于方法形成的理论基础对SRB浓度检测方法进行了分类,主要包括基于细菌培养、基于SRB代谢物质、基于遗传基因、基于特异性生物酶和基于生物传感器5大类;并分别选取具有代表性的绝迹稀释法、同位素标记法、PCR技术、酶联免疫吸附法和电化学传感器等经典方法,重点阐述各类方法的操作要领和应用特征。其次基于SRB的腐蚀机理,探讨了形成SRB腐蚀活性连续监测方法的理论基础,据此将监测方法分为基于生物膜演变和基于代谢产物积累两类方法;并针对目前已形成的电化学监测方法和硫离子选择电极法阐述了研究进展,剖析了实际应用中存在的问题。最后结合油气田对SRB腐蚀评价和控制的实际需求,对SRB浓度检测和腐蚀活性监测方法的发展趋势进行了展望。A variety of methods have been developed for concentration detection and corrosion activity monitoring of sulfate reducing bacteria(SRB)at home and abroad,but the application conditions of which are different.In this paper,the research progress of SRB concentration detection methods and corrosion activity monitoring methods was described in detail.At first,the methods for SRB concentration detection were classified based on the theoretical basis of the method formation,which mainly included five categories:bacteria cultivation,metabolites of SRB,genetic genes,specific biological enzyme and biosensor respectively.Some of the classical methods,including extinction dilution method,isotope-signed method,polymerase chain reaction,enzyme-linked immunosorbent assay and electrochemical biosensor,were selected respectively,and the operation essentials and application characteristics of each method were emphasized.Then,based on the corrosion mechanism of SRB,the theoretical basis of corrosion activity SRB monitoring technology was discussed.And the monitoring methods were divided into two groups:biofilm evolution and metabolite accumulation.The research progress of electrochemical monitoring method and sulfur ion selective electrode method was discussed and the problems existing in practical application were analyzed.Finally,the development tendency of SRB concentration detection and corrosion activity monitoring methods was prospected.

关 键 词：硫酸盐还原菌 检测方法 腐蚀活性 细菌代谢 

分 类 号：TE991[石油与天然气工程—石油机械设备] X835[环境科学与工程—环境工程]