基于转录组测序分析阿氏芽孢杆菌R60对玉米抗旱性的影响  

Effects of Bacillus aryabhattai R60 on drought tolerance of maize based on transcriptome sequencing analysis

作　　者：舒健虹[1] 王子苑 曾庆飞[1] 李小冬[1,2] 陈才俊 王小利[1] SHU Jian-hong;WANG Zi-yuan;ZENG Qing-fei;LI Xiao-dong;CHEN Cai-jun;WANG Xiao-li(Guizhou Institute of Prataculture,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China;Guizhou Dingxin Agriculture and Animal Husbandry Technology Co.,Ltd.,Guiyang,Guizhou 550006,China)

机构地区：[1]贵州省农业科学院草业研究所,贵州贵阳550006 [2]贵州鼎芯农牧科技有限公司,贵州贵阳550006

出　　处：《南方农业学报》2023年第8期2340-2351,共12页Journal of Southern Agriculture

基　　金：贵州省科技支撑计划项目(〔2019〕2359);贵州省科技计划项目(黔科合平台人才〔2020〕5005)。

摘　　要：【目的】分析干旱胁迫下阿氏芽孢杆菌(Bacillus aryabhattai)R60菌株对玉米耐旱性的影响,为抗旱性生物菌肥挖掘及玉米抗逆性提高提供理论参考。【方法】利用转录组测序技术,比较分析干旱胁迫下玉米根系接种阿氏芽孢杆菌R60菌株后的转录水平变化,以玉米根部注入灭菌的蒸馏水为对照,挖掘玉米的关键差异表达基因(DEGs),对其进行GO功能注释及KEGG信号通路富集分析。【结果】处理组和对照组共检测出1524个DEGs,与对照组相比,处理组有421个DEGs表达上调,有1103个DEGs表达下调。421个上调表达的DEGs共注释到26个GO条目上,包括13个生物学过程条目(细胞途径、代谢途径、刺激响应和生物调节等)、3个细胞成分条目(细胞结构体、细胞内和蛋白复合体)和10个分子功能条目(结合、催化活性和转录调控活性等)。表达下调程度排名前20的DEGs主要注释到5个生物学过程条目(细胞途径、生物调节和刺激响应等)、2个细胞成分条目(细胞结构体和细胞内)和4个分子功能条目(结合、转录调控活性等)。DEGs主要富集在RNA运输、mRNA通路、植物激素信号转导、丝裂原活化蛋白激酶(MAPK)信号通路及植物—病原互作等5条信号通路中。与对照组相比,处理组植物激素信号转导途径中,茉莉酸(JA)信号通路中的负调控茉莉酸ZIM结构域蛋白(JAZ)和下游的MYC2家族转录因子、赤霉素(GA)信号通路中的GA受体1(GID1)和负调控抑制蛋白(DELLA)、脱落酸(ABA)信号通路中的蛋白磷酸酶(PP2C)及乙烯信号通路中的乙烯受体转录因子1(ERF1)等编码基因表达下调;MAPK信号通路中,MAP激酶底物1(MKS1)和丝裂原活化蛋白激酶(MPK3、MKK9和MAPKKK18)等编码基因表达下调。此外,钙调节蛋白(CALM)和钙结合蛋白(CML)基因下调表达,而E3泛素连接酶(RHF、HOS1和BRE1)、磷酸核糖焦磷酸激酶(PRPS)和UDP葡萄糖醛酸酯4异构酶(GAE4)等编码基因表达上调。�【Objective】In this study,the effects of Bacillus aryabhattai R60 strain on drought tolerance of maize under drought stress were analyzed,which provided theoretical reference for drought tolerant biologic fertilizer exploration and improvement of maize stress tolerance.【Method】Transcriptome sequencing technology was used to analyze the changes in transcription levels of maize root inoculated with B.aryabhattai R60 strain under drought stress.Key differentially expressed genes(DEGs)in maize were explored,with sterilized distilled water injected into the root as the control,and GO function annotation and KEGG signaling pathway enrichment analysis were performed.【Result】There were 1524 DEGs between the treatment group and the control group.Compared with control group,there were 421 up-regulated DEGs and 1103 down-regulated DEGs in the treatment group.The 421 up-regulated DEG swere annotated to 26 GO entries,including 13 entries of biological processes(cellular pathways,metabolic pathways,stimulus response,and biological regulation,etc),3 entries of cell components(cellular structures,intracellular and protein complexes),and 10 entries of molecular functions(binding,catalytic activities and transcriptional regulatory activities,etc).The top of 20 DEGs with down-regulated expression were mainly annotated in 5 entries of biological processes(cellular pathways,biological regulation and stimulus response,etc),2 entries of cell components(cell structures and intracellular),and 4 entries of molecular functions(binding,transcriptional regulatory activities,etc).DEGs were mainly enriched in five signaling pathways of RNA transport,messenger mRNA pathway,plant hormone signal transduction,mitogen-activated protein kinase(MAPK)signaling pathway and plant-pathogen interaction.Compared with the control group,the expressions of key genes were down-regulated in the plant hormone signal transduction pathways of the treatment group,such as the negative regulation jasmonate ZIM domain-containing protein(JAZ)and downstream M

关 键 词：玉米 干旱胁迫 阿氏芽孢杆菌 耐旱性 转录组 

分 类 号：S513.035.3[农业科学—作物学]