枯草芽孢杆菌发酵甘草渣产2,3-丁二醇和乙偶姻  

Fermentation of 2,3-butanediol and acetoin from liquorice residue using Bacillus subtilis

作　　者：肖志伟 周克蒙 安雨萌 戴建英[1] 修志龙[1] 党艳艳[2] 权春善 XIAO Zhiwei;ZHOU Kemeng;AN Yumeng;DAI Jianying;XIU Zhiong;DANG Yanyan;QUAN Chunshan(School of Bioengineering,Dalian University of Technology,Dalian 116024,Liaoning,China;School of Chemistry and Chemical Engineering,Shihezi University,Shihezi 832003,Xinjiang,China;Key Laboratory of Biotechnology and Resource Utilization of Ministry of Education,Dalian Minzu University,Dalian 116600,Liaoning,China)

机构地区：[1]大连理工大学生物工程学院,辽宁大连116024 [2]石河子大学化学化工学院,新疆石河子832003 [3]大连民族大学生物技术与资源利用教育部重点实验室,辽宁大连116600

出　　处：《精细化工》2023年第12期2682-2688,共7页Fine Chemicals

基　　金：新疆兵团科技攻关计划项目(S2019AB1568);生物技术与资源利用教育部重点实验室开放课题(KF2022010)

摘　　要：以2种稀碱(Na2CO3水溶液和NaOH水溶液,下同)及稀碱和醋酸乙醇胺离子液体混合液为溶剂对甘草渣进行预处理,考察不同碱浓度和预处理温度对甘草渣组成及酶解效果的影响。结果表明,质量分数为2%的NaOH水溶液在固液比(g∶mL,下同)为1∶10、100℃条件下预处理甘草渣1.5 h,木质素去除率达54.1%、纤维素回收率为77.2%;样品酶解24 h,葡萄糖得率可达53.5%,较预处理前甘草渣(10.6%)提高了4.0倍。并对预处理后的甘草渣进行高固酶解,在固液比3∶10、纤维素酶用量45 FPU/g甘草渣条件下酶解72 h,葡萄糖产量达到86.2 g/L、木糖产量为18.9 g/L。以此酶解液为碳源进行发酵,64 h后发酵液中2,3-丁二醇和乙偶姻产量为43.9 g/L,还原糖转化率为0.42 g/g;与对照组相比,酶解液更有利于菌体生长,生产强度提高,但转化率略低。Liquorice residue was pretreated with two dilute basic solutions(Na2CO3 aqueous solution and NaOH aqueous solution)and the mixtures of the above dilute basic solution and ethanolamine acetate,respectively.The effects of different base concentrations and pretreatment temperature on the composition and enzymatic hydrolysis of liquorice residue were studied.The results showed that the removal rate of lignin and cellulose recovery rate reached 54.1%and 77.2%,respectively,under the conditions of 2%(mass fraction)NaOH aqueous solution as solvent,solid-liquid ratio(g∶mL,the same below)1∶10,time 1.5 h and temperature 100℃.The glucose yield was 53.5%after 24 h enzymatic digestion,which was 4.0 times higher than that without pretreatment(10.6%).When the pretreated liquorice residue was subjected to high solid enzymatic digestion at a solid-liquid ratio of 3∶10 with an enzyme dosage of 45 FPU/gliquorice residue for 72 h,the yield of glucose and xylose reached 86.2 and 18.9 g/L,respectively.This hydrolysate was used as carbon source for fermentation,the production of 2,3-butanediol and acetoin was 43.9 g/L after 64 h,and the conversion rate of reducing sugar was 0.42 g/g.Compared with control group,the hydrolysate was more favorable to the growth of bacteria,while the productivity of 2,3-butanediol and acetoin was increased and the conversion rate was slightly lower.

关 键 词：甘草渣 预处理 碱溶液 高固酶解发酵 2 3-丁二醇 生物工程 

分 类 号：TQ452.1[化学工程—农药化工] X705[环境科学与工程—环境工程]