褪黑素抑制人血管平滑肌细胞的巨噬样表型转化  

Melatonin inhibits the macrophage-like phenotype transformation of human vascular smooth muscle cells

作　　者：李双 谭翠钰 侯连杰 赵国军 LI Shuang;TAN Cuiyu;HOU Lianjie;ZHAO Guojun(Dali University,Dali 671003,Yunnan,China;Qingyuan People's Hospital,the Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan 511518,Guangdong,China)

机构地区：[1]大理大学,云南大理671003 [2]广州医科大学附属第六医院清远市人民医院,广东清远511518

出　　处：《中南医学科学杂志》2023年第6期808-812,共5页Medical Science Journal of Central South China

基　　金：国家自然科学基金面上项目(81870337,82300517);广州医科大学2022年度学生创新能力提升计划项目(2022-66)。

摘　　要：目的探究褪黑素在人血管平滑肌细胞(VSMC)巨噬样表型转化中的作用。方法VSMC分为对照组、胆固醇组(水溶性胆固醇)、褪黑素组(水溶性胆固醇+褪黑素)。水溶性胆固醇诱导VSMC建立巨噬样表型转化模型。定量PCR检测VSMC巨噬样表型相关基因表达水平;油红O染色观察各组VSMC脂质吞噬能力;Edu染色观察各组VSMC增殖情况;细胞划痕实验观察VSMC迁移情况。结果10 mg/L水溶性胆固醇显著降低平滑肌收缩表型相关标志基因α-actin和α-tropomyosin mRNA表达,并显著升高巨噬细胞相关标志基因CD68和MAC2 mRNA表达,诱导VSMC巨噬样表型转化。0.5μmol/L褪黑素可抑制水溶性胆固醇引起的VSMC脂质蓄积和细胞增殖,但对VSMC迁移无影响。结论褪黑素抑制VSMC的巨噬样表型转化。Aim To establish a macrophage-like phenotypic transformation model of human vascular smooth muscle cells(VSMC)induced by water-soluble cholesterol,and to explore the role of melatonin in this process.Methods VSMC is divided into a control group,a cholesterol group(water-soluble cholesterol),and a melatonin group(watersoluble cholesterol+melatonin).Establishment of a macrophage like phenotype transformation model in VSMC induced by water-soluble cholesterol.The expression of macrophage-like phenotypic related genes in VSMC was detected by quantitative PCR.Oil red O staining experiment was conducted to observe the effect of melatonin on the lipid accumulation of VSMC induced by water-soluble cholesterol.Edu staining was used to observe the effect of melatonin on the proliferation of VSMC induced by water-soluble cholesterol.The effect of melatonin on VSMC migration induced by water soluble cholesterol was evaluated by cell scratch assay.Results The mRNA expression of smooth musclecontraction phenotype related markers,α-actin andα-tropomyosin,were significantly decreased after 10 mg/L water-soluble cholesterol treatment,while the mRNA expression macrophage related markers,CD68 and MAC2,were significantly increased after treated by 10 mg/L water-soluble cholesterol,suggesting the transformation of VSMC to a macrophage-like phenotype.In addition,0.5μmol/L melatonin inhibited lipid accumulation and proliferation of VSMC induced by water-soluble cholesterol,but had no effect on VSMC migration.Conclusion Melatonin inhibits the macrophage-like phenotype transformation of VSMC.

关 键 词：动脉粥样硬化 血管平滑肌细胞 巨噬样表型转化 褪黑素 

分 类 号：R967[医药卫生—药理学]