微流控芯片技术及质谱技术用于细菌耐药性检测及耐药机制研究  

Microfluidic chip and mass spectrometry-based detection of bacterial antimi⁃crobial resistance and study of antimicrobial resistance mechanism

作　　者：张冬雪 乔亮[1] ZHANG Dongxue;QIAO Liang(Department of Chemistry,and Institutes of Biomedical Sciences,Fudan University,Shanghai 200433,China)

机构地区：[1]复旦大学化学系,复旦大学生物医学研究院,上海200433

出　　处：《中国药科大学学报》2023年第6期695-705,共11页Journal of China Pharmaceutical University

基　　金：中国博士后科学基金资助项目(No.2022M720806)。

摘　　要：细菌耐药性严重影响全球公共卫生安全。抗生素错用和滥用不仅没有达到治疗细菌感染性疾病的效果,反而会刺激细菌发生DNA损伤修复反应(SOS反应),加剧细菌耐药性的进化和耐药菌的传播。本文聚焦于耐药菌,简明介绍细菌耐药性与SOS反应,系统概述了质谱技术、微流控技术及其联用技术在细菌检测及细菌耐药机制研究中的应用。本文为细菌耐药性相关的药物靶点挖掘及新药开发提供理论参考,以期发展细菌耐药性快速检测新方法和抑菌新方法,推动临床细菌感染性疾病的诊断与治疗。Bacterial antimicrobial resistance(AMR)is a globally serious problem that threatens public health security.Misuse and abuse of antibiotics cannot achieve the effect of treating bacterial infectious diseases,but will trigger the SOS response of bacteria,exacerbating the evolution of bacterial AMR and the spread of resistant bacteria.This article focuses on antibiotic-resistant bacteria,briefly introduces the pathogenesis of bacterial AMR and SOS response,and systematically summarizes the determination and mechanism study of bacterial AMR based on microfluidics and mass spectrometry.This article provides theoretical basis for AMR-related drug target mining and new drug development,aiming to develop new methods for rapid detection of bacterial AMR and new methods for bacteria inhibition,and promote the diagnosis and treatment of clinical bacteria infectious diseases.

关 键 词：细菌耐药 耐药机制 微流控技术 质谱检测 组学分析 

分 类 号：O65[理学—分析化学] R318[理学—化学]