荧光原位杂交法分析肥披碱草的染色体核型  

Chronosome Karyotype Analysis of Elymus excelsus Turcz.by Fluorescence in Situ Hybridization

作　　者：岳静宇 李景环[1] YUE Jingyu;LI Jinghuan(College of Life Science and Technology,Inner Mongolia Normal University,Hohhot 010022,China)

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010022

出　　处：《种子》2023年第10期65-69,76,共6页Seed

基　　金：内蒙古自治区高等学校科学研究项目(NJZY18024)。

摘　　要：以肥披碱草为材料,用45S rDNA和5S rDNA做探针,通过荧光原位杂交(FISH)法,借助肥披碱草染色体内45S rDNA、5S rDNA的分布特性,分析其染色体核型。结果表明,检测到45S rDNA中第2号、第20号染色体短臂的末端及第17号染色体短臂次缢痕上有杂交信号,并检测到5S rDNA中第2号、第7号和第14号染色体短臂上及第17号染色体短臂次缢痕上有杂交信号。肥披碱草的核型公式为2n=6x=42=30 m+12 sm(4 sat);染色体平均长度为12.68μm,染色体长度范围为9.19~17.39μm,染色体组总长度为266.29μm,根据Stebbins核型分类原则,肥披碱草核型属于2A型。Using Elymus excelsus Turcz.as the material and 45S rDNA and 5S rDNA as probes,through the distribution characterics in chromosome of 45S rDNA and 5S rDNA,of which the chromosomal karyotypes were analyzed by fluorescence in situ hybridization(FISH).The results showed that hybridization signals were detected on the end of short arm of chromosome 2 and chromosome 20 and on the secondary constriction of short arm of chromosome 17 in 45S rDNA.Hybridization signals were detected on the short arm of chromosomes 2,7 and 14 and on the secondary constriction of the short arm of chromosome 17 in 5S rDNA.The karyotype formula was 2n=6x=42=30 m+12 sm(4 sat).The average length of chromosomes was 12.68μm,the length of chromosomes ranged from 9.19 to 17.39μm,and the total length of chromosomes was 266.29μm.According to the principle of Stebbins karyotype classification,the karyotype of the Elymus excelsus Turcz.was 2A.

关 键 词：肥披碱草 荧光原位杂交 45S rDNA 5S rDNA 核型 

分 类 号：S543.9[农业科学—作物学]