荞麦属五种植物叶绿体基因组的结构变异与分子进化研究  

Structural Variation and Molecular Evolution of Chloroplast Genomes in Five Species of Fagopyrum

作　　者：黄超婷 孙豪良 盛文涛[1] HUANG Chaoting;SUN Haoliang;SHENG Wentao(Department of Biological Technology,Nanchang Normal University,Nanchang 330032,China)

机构地区：[1]南昌师范学院生物技术研究院,南昌330032

出　　处：《种子》2023年第10期83-88,98,共7页Seed

基　　金：江西省自然科学基金(20171BAB214024,20202BABL203044);江西省科技厅科技合作专项(20212BDH81022);江西省教育厅科技项目(GJJ202619);南昌师范学院教学研究立项课题(JXJG-22-23-3,NSJG-21-25);江西省油料作物生物学重点实验室开放基金(YLKFKT202203)。

摘　　要：利用生物信息学方法对荞麦属叶绿体进行比较基因组学研究。结果表明,荞麦属已测的5种植物叶绿体基因组大小在159265~159599 bp之间;GC含量介于37.81%~37.98%之间,基因的数目、类型与拷贝数差异小;区域边界基本一致;SSR类型的种类与数目相似;该属的LSC区域和SSC区域比IR区域多态性位点变异高,其中非编码区trnS_trnG、rps4_trnT和rpl32_trnL是变异最高的热点区域;系统分析表明,野生金荞麦与苦荞的亲缘关系最近。In this study,comparative genomics of Fagopyrum chloroplasts was studied using bioinformatics methods.The results showed that the chloroplast genome sizes of the five species of Fagopyrum ranged from 159265 to 159599 bp;the GC content ranged from 37.81%to 37.98%,and there was a small difference in the number,type,and copy number of genes;The regional boundaries were basically consistent;The type and number of SSR types were similar;The polymorphism of LSC and SSC in this genus was higher than that in IR region,and the non-coding regions trnS-trnG,rps4-TrnT and rpl32-TrnL were the hot-spot region with the highest variation;Systematic analysis showed that F.leptopodum had the closest genetic relationship with F.tataricum.

关 键 词：荞麦属 叶绿体 基因组特征 

分 类 号：S517[农业科学—作物学]