基于UHPLC⁃Q⁃Orbitrap/MS的不同产地西洋参皂苷类成分分析  

UHPLC-Q-Orbitrap/MS-based analysis of saponins from Panacis Quinquefolii Radix of different origins

作　　者：梁力文 郭娜[1] 刘小康 黄鑫 蔡广知[1] 郭云龙 贡济宇[1] LIANG Li-wen;GUO Na;LIU Xiao-kang;HUANG Xin;CAI Guang-zhi;GUO Yun-long;GONG Ji-yu(College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China;Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117 [2]长春中医药大学吉林省人参科学研究院,吉林长春130117

出　　处：《中成药》2023年第12期4017-4024,共8页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81903778)。

摘　　要：目的基于UHPLC-Q-Orbitrap/MS分析不同产地(吉林、辽宁、黑龙江、山东)西洋参皂苷类成分。方法运用UHPLC-Q-Orbitrap/MS法定性分析西洋参中皂苷类成分,结合主成分分析、正交偏最小二乘法判别分析、差异成分分析筛选各产地的差异性皂苷。结果共得到62个皂苷类化合物。主成分分析表明不同产地西洋参中所含皂苷类成分存在差异,正交偏最小二乘判别分析进一步筛选出28个差异皂苷类化合物,包括原人参二醇型13个、原人参三醇型6个、齐墩果烷型4个、奥克梯隆型2个、C-17侧链变异型3个;原人参二醇型和原人参三醇型皂苷类成分在4个产地相对含量由高到低依次为辽宁、吉林、黑龙江、山东。结论该方法简便、准确、高效,可为西洋参的质量评价提供理论依据。AIM To analyze saponins from Panacis Quinquefolii Radix of different regions(Jilin,Liaoning,Heilongjiang,Shandong)based on UHPLC-Q-Orbitrap/MS technology.METHODS Panacis Quinquefolii Radix had its s aponins qualitatively analyzed by UHPLC-Q-Orbitrap/MS;and its differential saponins revealing different regions screened by the principal component analysis,orthogonal partial least squares discriminant analysis and differential component analysis.RESULTS A total of 62 saponins were identified;saponin variations due to the growth areas verified by the principal component analysis;and 28 differential saponins including 13 protopanaxadiol,6 protopanaxatriol,4 oleanolane,2 oxytetracycline,and 3 C-17 side chain variants further screened by orthogonal partial least-squares discriminant analysis model.The relative contents of protopanaxadiol-type and protopanaxatriol-type saponins in the four producing areas reduced following the order of Liaoning,Jilin,Heilongjiang,and Shandong.CONCLUSION This simple,accurate and efficient method provides a theoretical basis for the quality evaluation of Panacis Quinquefolii Radix.

关 键 词：西洋参 皂苷 主成分分析 正交偏最小二乘法判别分析 差异成分分析 UHPLC-Q-Orbitrap/MS 

分 类 号：R284.1[医药卫生—中药学]