小蓟TLC鉴别方法优化及小蓟炭“炒炭存性”内在表征研究  

Optimization of TLC identification for Cirsii Herba and research on changes in quality properties“carbonizing retains characteristics”of its charred product

作　　者：卢琪 张天泽 朱娟娟 曲珍妮 徐春梦 戴衍朋[1,2] 石典花 LU Qi;ZHANG Tian-ze;ZHU Juan-juan;QU Zhen-ni;XU Chun-meng;DAI Yan-peng;SHI Dian-hua(Shandong Academy of Chinese Medicine,Jinan 250014,China;The SATCM Key Research Lab for the Technology and Principles in Honey-Processing and Carbonizing of Chinese Materia Medica,Jinan 250014,China;Beijing University of Chinese Medicine,Beijing 100105,China;Shandong University of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东省中医药研究院,山东济南250014 [2]国家中医药管理局中药蜜制和制炭炮制技术与原理重点研究室,山东济南250014 [3]北京中医药大学,北京100105 [4]山东中医药大学,山东济南250014

出　　处：《中成药》2023年第12期4024-4029,共6页Chinese Traditional Patent Medicine

基　　金：国家中医药管理局中药炮制技术传承基地建设项目(2022);山东省自然基金(ZR2023MH114);山东省中医药科技发展计划项目(Q-2022085,Z-2022086T);山东省中医药研究院孵化基金(2021SACM-2);山东省中医药特色疗法挖掘整理推广应用项目(2021);山东省中医药高层次人才培育项目(鲁卫中医药科教字[2021]6号);齐鲁医派中医学术流派传承项目(2021)。

摘　　要：目的优化小蓟TLC鉴别方法并研究小蓟炭“炒炭存性”内在表征。方法对2020年版《中国药典》中“小蓟”项下TLC展开方法中的薄层板和展开剂进行改进,建立小蓟炭的TLC鉴别方法和UPLC指纹图谱,并测定蒙花苷、金合欢素的含量。结果小蓟TLC鉴别建议使用硅胶G板,展开剂建议改为甲苯-丙酮-甲酸-甲醇(6∶3∶0.5∶2.5)。小蓟及不同炒制程度小蓟炭的蒙花苷、金合欢素含量变化与TLC鉴别结果一致。结论与2020年版《中国药典》相比,改进的小蓟TLC鉴别方法较原方法成本低,所用展开剂毒性小。建立的小蓟炭“炒炭存性”的内在表征结合外观性状可用于解决市售小蓟炭的质量问题。AIM To optimize the TLC identification method for Cirsii Herba and to study the changes in quality properties“carbonizing retains characteristics”of Cirsii Herba Carbonisata.METHODS After the improvement of thin layer plate and solvent in the TLC identification based on the Chinese Pharmacopoeia 2020 Edition for Cirsii Herba,Cirsii Herba Carbonisata had its TLC identification method and UPLC fingerprint established for the determination of its content of buddleoside and acacetin as well.RESULTS We used silica gel G plate,and the solvent of toluene-acetone-formic acid-methanol(6∶3∶0.5∶2.5)for TLC identification of Cirsii Herba.The content variations of buddleoside and acacetin in Cirsii Herba and its differently charred products were consistent with the result of the TLC identification.CONCLUSION The improved TLC identification method for Cirsii Herba is of lower cost and less solvent toxicity compared to the method in the Chinese Pharmacopoeia 2020 Edition,and can identify the changes in quality properties“carbonizing retains characteristics”of differently charred Cirsii Herba,therefore it can be used to control the quality of Cirsii Herba Carbonisata in the market.

关 键 词：小蓟 小蓟炭 TLC 炒炭存性 

分 类 号：R283[医药卫生—中药学]