孵育系统中的微孔板支架结构优化  

Optimization of microplate holder structures in incubation system

作　　者：姜容 陈正伟 樊伟东 骆志成 黄俊[1] JIANG Rong;CHEN Zhengwei;FAN Weidong;LUO Zhicheng;HUANG Jun(School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023,Zhejiang,China;Hangzhou Allsheng Instrument Co.,Ltd.,Hangzhou 310023,Zhejiang,China)

机构地区：[1]浙江科技学院生物与化学工程学院,杭州310023 [2]杭州奥盛仪器有限公司,杭州310012

出　　处：《浙江科技学院学报》2023年第6期495-503,526,共10页Journal of Zhejiang University of Science and Technology

基　　金：国家重点研发计划项目(2021YFF0600805);浙江省重点研发计划项目(2022C02049);浙江科技学院青年科学基金项目(2021QN072)。

摘　　要：【目的】为提高多功能微孔板读数仪的孵育性能,针对微孔板温度均一性,提出微孔板支架组件的结构改进方案。【方法】首先分析影响微孔板读数仪孵育性能的因素,包括支架组件的结构尺寸、表面处理和加工材料等,并设计改进方案;然后通过多通道数据采集仪进行试验,收集不同方案的微孔板支架组件孵育时微孔板中各孔位的实际温度;最后,对比不同改进方案在最大温差、升温速率等孵育性能方面的提升程度,评价各改进方案的孵育性能并确定最佳改进方案。【结果】相比改进前的支架组件结构,与微孔板底部完全接触的支架组件改进方案在试验中的表现最佳:各孔位间最大温差从2.9℃降至1.1℃,降低了62.07%;升温过程中最大温差超过1℃的持续时间从1650 s降至120 s,降低了92.72%;前7 min平均升温速率从0.774℃/min升至1.065℃/min,提升了37.60%;前7 min平均升温速率标准差从0.100降至0.043,降低了57.00%。【结论】本优化设计提供了一种可行的微孔板支架组件结构改进思路,对提升微孔板读数仪的孵育性能具有一定的参考价值。[Objective]Aiming at the temperature uniformity of the microplate,a structural improvement scheme of the microplate holder assembly was proposed to improve the incubation performance of the multi-functional microplate reader.[Method]Firstly,factors affecting the incubation performance of the microplate reader were analyzed,including structural size,surface treatment and processing materials of the holder components,and then improvement schemes were designed;secondly,the multi-channel data acquisition instrument was used to test and collect the actual temperature of each well in the microplate when the microplate holder components of different protocols were incubated;finally,the improvement degree of the incubation performance of different improvement schemes were compared in terms of maximum temperature difference and heating rate,and the evaluation of the incubation performance of each improvement scheme was completed.[Result]Compared with the original structure of the holder assembly,the modified holder assembly with full contact with the bottom of the microplate performs best in the test:the maximum temperature difference at each well position decreases from 2.9℃to 1.1℃by 62.07%;during the heating process,the duration of the maximum temperature difference over 1℃decreases from 1650 s to 120 s by 92.72%;the average heating rate in the first 7 min increases from 0.774℃/min to 1.065℃/min by 37.60%;the standard deviation of the average heating rate in the first 7 min decreases from 0.100 to 0.043 by 57.00%.[Conclusion]This optimization design provides a feasible way to improve the structure of the microplate holder assembly,which has certain reference for improving the incubation performance of the microplate reader.

关 键 词：微孔板读数仪 微孔板支架 热传导 接触热阻 升温曲线 

分 类 号：TB472[一般工业技术—工业设计]