两株麦冬内生细菌的鉴定及生物学特性研究  

Identification and Biological Characteristicsof Two Endophytic Bacterias from Ophiopogon Japonicus

作　　者：沈瑜琦 陈帅 李维波 牛然 邵登科 张春源 赖泽成 叶文雨 SHEN Yuqi;CHEN Shuai;LI Weibo;NIU Ran;SHAO Dengke;ZHANG Chunyuan;LAI Zecheng;YE Wenyu(National Engineering Research Center of JUNCAO Technology,Fuzhou Fujian 350002,China;Fujian University Key Laboratory for Plant-Microbe Interaction,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China)

机构地区：[1]国家菌草工程技术研究中心,福建福州350002 [2]植物与微生物相互作用福建省高校重点实验室,福建农林大学生命科学学院,福建福州350002 [3]福建农林大学植物保护学院,福建福州350002

出　　处：《青海农林科技》2023年第4期1-5,21,共6页Science and Technology of Qinghai Agriculture and Forestry

基　　金：福建省引导性科技计划项目(2022N0010);福建省自然资源科技创新项目(KY-090000-04-2022-016);福建农林大学科技创新专项基金项目(KFA19137A);福建农林大学科技创新专项基金项目(KFA19136A);福建省科技重大专项(2021NZ029009)。

摘　　要：本研究从麦冬健康叶片中分离得到两株内生细菌,并对其进行了分子和生理生化特性鉴定。结果表明:MD1菌株是路德维希肠杆菌(Enterobacter ludwigii),淀粉水解试验、甲基红试验和过氧化氢酶试验均为阳性;MD2菌株是节杆菌(Arthrobacter woluwensis),淀粉水解试验和过氧化氢酶试验均为阳性,而甲基红试验为阴性。本结果为麦冬内生细菌的功能菌株的筛选和多样性研究提供理论基础。In this study,two endophytic bacterias,were isolated from healthy leaves of Ophiopogon japonicus and identified molecularly and physiologically and biochemically.The results showed that the strain MD1 was identified as Enterobacter ludwigii and the starch hydrolysis test,the methyl red test and the catalase test were all positive.While the strain MD2 was identified as Arthrobacter woluwensis,and the starch hydrolysis test and the catalase test were both positive,while the methyl red test showed negative result.The results of this study provide a scientific basis for further research on the diversity of Ophiopogon japonicus endophytic bacterias and lay a foundation for the future screening of functional bacterias of Ophiopogon japonicus endophytes.

关 键 词：麦冬 内生细菌 生物学特性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]