基于TRM70303发酵产物硫藤黄菌素的乳油制备  

Preparation of emulsifiable concentrate based on TRM70303fermentation product thiolutin

作　　者：宋迎 何沐昕 张阿郎 刘蕊琪 王丽君[1] 夏占峰[1] SONG Ying;HE Muxin;ZHANG A’lang;LIU Ruiqi;WANG Lijun;XIA Zhanfeng(College of Life Science and Technology,Tarim University State Key Laboratory Breeding Base for The Protection and Utilization of Biological Resources in Tarim Basin Co-funded by Xinjiang Production and Construction Corps and The ministry of Science and Technology,Alar,Xinjiang 843300)

机构地区：[1]塔里木大学生命科学与技术学院/新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室-省部共建国家重点实验室培育基地,新疆阿拉尔843300

出　　处：《塔里木大学学报》2023年第4期8-15,共8页Journal of Tarim University

基　　金：新疆生产建设兵团财政科技计划资助项目“塔里木盆地来源微生物防治新疆主要农林病害菌剂的开发和应用”(2021BC009);国家级大学生创新创业训练计划项目“基于塔里木盆地高抑菌活性放线菌发酵产物的生物农药创制”(202210757018)。

摘　　要：因化学农药制剂对植物和土壤生态的环境污染问题,开发生物源农药用于防治农业病害成为发展趋势。本研究通过以微生物来源的天然抗菌物质研制对棉花黄萎病具有高效、低毒、低残留以及使用方便等特点的乳油制剂,以期为新疆棉花产业的健康发展提供支持。本研究以塔里木盆地放线菌Streptomyces sp. TRM70303的主要活性产物硫藤黄菌素为原料,探讨制备硫藤黄菌素乳油的最佳配方。采用菌丝生长速率法初步测定硫藤黄菌素对大丽轮枝菌的皿内抑菌活性;通过对溶剂和乳化剂进行配方优化筛选,得到硫藤黄菌素乳油最佳配方为硫藤黄菌素原药10%,农乳500#12%,DMSO补足至100%。Due to the chemical pesticide preparation,pollution on plant and soil ecology,the development of biological pesticides to control agricultural diseases become a trend.In order to provide support for the healthy development of cotton industry in Xinjiang,emulsifiable concentrate with high efficiency,low toxicity,low residue and convenient use was developed by using natural products with high antibacterial activity from microbial sources.The preparation method of emulsifiable concentrate fungicide was studied by using the main active compound of Streptomyces sp.TRM70303 from Tarim Basin as a raw material in the study.The antifungal activity of thiolutin against Verticillium dahliae was preliminarily determined by mycelial growth rate method;By screening and optimizing the solvent and emulsifier,the best formula of thiolutin emulsifiable concentrate was composed of 10%thiolutin,12%Nongru 500#,and DMSO supplemented to 100%.

关 键 词：硫藤黄菌素 乳油 棉花黄萎病 

分 类 号：Q939.11[生物学—微生物学]