PLIN2通过Rab18上调ACSL3表达促进巨噬细胞脂质蓄积  

PLIN2 promotes lipid accumulation in macrophages by up-regulating ACSL3 expression through Rab18

作　　者：张星星 张荣 杨丽[1] 刘兰 罗健雄 王语婷 胡博雅 袁中华[1,2] ZHANG Xingxing;ZHANG Rong;YANG Li;LIU Lan;LUO Jianxiong;WANG Yuting;HU Boya;YUAN Zhonghua(Institute of Cardiovascular Disease,University of South China&Key Laboratory of Arterial Hard Chemistry of Hunan Province&Hunan Province Arteriosclerotic Disease International Scientific and Technological Innovation Cooperation Base;Department of Pharmacology,School of Pharmacy,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学心血管疾病研究所,动脉硬化学湖南省重点实验室,湖南省动脉硬化性疾病国际科技创新合作基地 [2]南华大学药学院药理学教研室,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2023年第12期1020-1028,共9页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金项目(81973326);湖南省教育厅科学研究项目(21C0283,21C0290)。

摘　　要：[目的]探讨脂滴包被蛋白2(PLIN2)增加巨噬细胞内脂质蓄积的机制。[方法]将实验分为氧化型低密度脂蛋白(ox-LDL)组、不同PLIN2表达组、不同活性Rab18组,测定高表达和沉默表达PLIN2巨噬细胞中的Rab18和脂酰辅酶A长链合成酶3(ACSL3)蛋白水平,不同活性Rab18的高表达PLIN2巨噬细胞中的PLIN2、Rab18、ACSL3蛋白表达水平。采用Western blot检测蛋白表达水平,采用免疫荧光观察细胞内相关蛋白定位情况,采用油红O染色观察细胞内脂质蓄积情况。[结果]高表达PLIN2的细胞中Rab18、ACSL3表达水平明显增加(P<0.05),且PLIN2与Rab18、ACSL3在细胞内存在共定位的现象。转染Rab18显性突变体Q67L质粒(Rab18活性增强)后的PLIN2高表达巨噬细胞中ACSL3表达水平明显升高(P<0.05),细胞内脂滴的数量也明显增多(P<0.05)。[结论] PLIN2可通过Rab18上调ACSL3表达促进巨噬细胞脂质蓄积。Aim To investigate the mechanism of perilipin 2(PLIN2)increasing lipid accumulation in macrophages.Methods The experiments were divided into oxidized low density lipoprotein(ox-LDL)group,different PLIN2 expression groups,and different activity Rab18 groups,the levels of Rab18 and acyl-CoA long chain synthetase 3(ACSL3)protein in high expression and silent expression of PLIN2 macrophages were measured,and the levels of PLIN2,Rab18,and ACSL3 protein expression in high expression PLIN2 macrophages with different activity Rab18 were measured.Western blot was used to detect protein expression levels,immunofluorescence was used to observe the localization of intracellular related proteins,and oil red O staining was used to observe intracellular lipid accumulation.Results The expression levels of Rab18 and ACSL3 in cells with high expression of PLIN2 were increased significantly(P<0.05),and there was an intracellular phenomenon of co-localization of PLIN2 with Rab18 and ACSL3.The expression level of ACSL3 in high expression macrophages of PLIN2 after transfection with the Rab18 dominant mutant Q67L plasmid(Rab18 activity increased)was increased significantly(P<0.05),and the number of intracellular lipid droplets was also increased significantly(P<0.05).Conclusion PLIN2 promotes macrophage lipid accumulation through Rab18 by up-regulating ACSL3.

关 键 词：脂滴包被蛋白2 Rab18 脂酰辅酶A长链合成酶3 脂质蓄积 

分 类 号：R5[医药卫生—内科学] R363[医药卫生—临床医学]