赤霉素和氯吡脲的体外细胞及致突变毒性研究  

A Study on in vitro cell and mutagenic toxicity of gibberellin and forchlorfenuron

作　　者：图尔逊娜依·艾力 吴军强 雒怡倩 王潇 杨洁[1] 岳海涛[1] AILI·Tuerxunnayi;WU Junqiang;LUO Yiqian;WANG Xiao;YANG Jie;YUE Haitao(College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang 830046,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《石河子大学学报（自然科学版）》2023年第6期730-736,共7页Journal of Shihezi University(Natural Science)

基　　金：国家重点研发计划食品安全关键技术研发重点专项子课题(2019YFC1606102)。

摘　　要：赤霉素和氯吡脲作为高效的植物生长调节剂,被广泛用于水果果实的膨大和无核化生产中。为探究赤霉素和氯吡脲接触细胞后所发生的抑制细胞生长和致突变毒性作用,评价赤霉素和氯吡脲的损伤,利用人正常肝细胞(WRL68)对赤霉素和氯吡脲在细胞水平毒性做初步探究,通过小鼠淋巴瘤细胞(L5178Y)TK基因突变试验、鼠伤寒沙门氏菌Ames试验对赤霉素和氯吡脲致突变性进行评价。研究结果表明,赤霉素和氯吡脲对WRL68细胞的半数抑制浓度分别为1.377 mg·mL^(-1)和57.72μg·mL^(-1);赤霉素在4 mg·mL^(-1)、氯吡脲在100μg·mL^(-1)剂量下,未观察到致突变毒性。赤霉素和氯吡脲虽不同程度的抑制WRL68细胞增殖但是不存在致突变毒性。Gibberellin and forchlorfenuron,as efficient plant growth regulators,are widely used in fruit enlargement and seedless production.In order to explore the inhibition of cell growth and mutagenic toxicity of gibberellic acid and forchlorfenuron after contact with cells,and to evaluate the damage of gibberellic acid and forchlorfenuron,the cytotoxicity of gibberellic acid and forchlorfenuron at the cellular level was preliminarily explored by using human normal liver cells(WRL68).The mutagenicity of gibberellic acid and forchlorfenuron was evaluated by TK gene mutation test of mouse lymphoma cells(L5178Y)and Ames test of Salmonella typhimurium.The research results indicated that the IC_(50)of GA and forchlorfenuron on WRL68 cells were 1.377 mg·mL^(-1)and 57.72μg·mL^(-1),respectively.Meanwhile,No mutagenic toxicity was observed that at 4 mg·mL^(-1)gibberellin and 100μg·mL^(-1)forchlorfenuron of dosage.Gibberellin and forchlorfenuron inhibited the proliferation of WRL68 cells in varying degrees,but there was no mutagenic toxicity.

关 键 词：赤霉素 氯吡脲 细胞毒性 致突变毒性 AMES试验 

分 类 号：S482.8[农业科学—农药学] TS201.6[农业科学—植物保护]