金银花黑斑病病原菌番茄匍柄霉的分离与鉴定  

Isolation and Identification of Pathogenic Fungus Stemphylium lycopersici Causing the Black Spot Disease on Honeysuckle(Lonicera japonica Thunb.)

作　　者：刘端冲 杨金库[2] 林若竹 姚艳霞[1] 淮稳霞[1] 赵文霞[1] LIU Duan-chong;YANG Jin-ku;LIN Ruo-zhu;YAO Yan-xia;HUAI Wen-xia;ZHAO Wen-xia(Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Ecology and Nature Conservation Institute,Chinese Academy of Forestry,Beijing 100091,China;Langfang Academy of Agriculture and Forestry Sciences,Langfang 065000,Hebei,China)

机构地区：[1]国家林业和草原局森林保护学重点实验室,中国林业科学研究院森林生态环境与自然保护研究所,北京100091 [2]廊坊市农林科学院,河北廊坊065000

出　　处：《林业科学研究》2023年第6期30-39,共10页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项“塞罕坝重大林业灾害防控技术研究”(项目编号CAFYBB2018ZB005)。

摘　　要：[目的]分离并鉴定引起金银花黑斑病的病原菌,为该病害的有效防控提供理论依据。[方法]采用常规组织分离法对发病金银花叶片进行分离,结合形态学、多基因序列分析(ITS、gpd和EF-1α序列)和致病性测定进行病原物种鉴定。[结果]在形态学上,分离获得的菌株YP4在PDA培养基上的菌落呈灰白或黄白色棉毛状,与番茄匍柄霉的菌落特征相符;在基于ITS、gpd和EF-1α序列联合构建的3种系统发育树中,供试菌株YP4均以较高支持率与番茄匍柄霉(Stemphylium lycopersici)聚在同一分支上;致病性接种实验结果表明,菌株YP4接种至金银花叶片上和番茄叶片上均可产生病斑,并可再分离获得其纯菌株;因此,结合形态学、分子系统发育学以及致病性测定,该病原真菌被鉴定为番茄匍柄霉。[结论]本研究明确了引起河北省金银花黑斑病的病原菌为番茄匍柄霉,这是该病菌在我国危害金银花的首次报道。[Objective]To clarify the pathogen causing black spot disease on Lonicera japonica,and to provide a theoretical basis for effective prevention and control of this disease.[Method]The strains of the pathogen were isolated by conventional tissue plate method,and were identified by the morphological characters and phylogenetic analysis of ITS,gpd and EF-1αsequences.Its pathogenicity was tested through in vitro inoculating healthy leaves of L.japonica and Solanum lycopersicum.[Result]The isolated fungal strain YP4 morphologically formed greyish to yellowish white,cottony colonies on Potato Dextrose Agar,which were consistent with published descriptions of Stemphylium lycopersici.In the three phylogen-etic trees based on ITS,gpd and EF-1αsequences,the isolate YP4 fell into the S.lycopersici clade with strong support.Based on the pathogenicity measurement of Koch's rule,the strain YP4 was pathogenic to leaves of L.japonica and S.lycopersicum.[Conclusion]Combined with the morphological characteristics,the pathogenicity determination and phylogenetic analysis of ITS,gpd and EF-1αsequences,the patho-gen causing black spot disease on honeysuckle is identified as S.lycopersici.To our knowledge,this is the first report of S.lycopersici causing black spot of L.japonica in China.

关 键 词：金银花 黑斑病 番茄匍柄霉 

分 类 号：S763.15[农业科学—森林保护学]