南亚热带乡土阔叶林与马尾松林土壤细菌群落多样性和结构  

Diversity and Structure of Soil Bacterial Communities in Native Broadleaved Plantations and Pinus massoniana Plantation in Southern Subtropical China

作　　者：滕金倩 肖指柔 明安刚[2,3] 朱豪 席守鸿 何江 朱茂锋 陶怡 覃林[1] TENG Jin-qian;XIAO Zhi-rou;MING An-gang;ZHU Hao;XI Shou-hong;HE Jiang;ZHU Mao-feng;TAO Yi;QIN Lin(Guangxi Key Laboratory of Forest Ecology and Conservation,College of Forestry,Guangxi University,Nanning 530004,Guangxi,China;Experiment Center of Tropical Forestry,Chinese Academy of Forestry,Pingxiang 532600,Guangxi,China;Guangxi Youyiguan Forest Ecosystem Research Station,Pingxiang 532600,Guangxi,China)

机构地区：[1]广西大学林学院,广西森林生态与与保育重点实验室,广西南宁530004 [2]中国林业科学研究院热带林业实验中心,广西凭祥532600 [3]广西友谊关森林生态系统定位观测研究站,广西凭祥532600

出　　处：《林业科学研究》2023年第6期58-68,共11页Forest Research

基　　金：国家自然科学基金项目(31560109、32071764);广西自然科学基金项目(2020GXNSFAA297208)。

摘　　要：[目的]探究针叶林转换成阔叶林后土壤细菌群落多样性和结构的变化特征。[方法]以我国南亚热带地区针叶马尾松林及乡土阔叶红锥林、米老排林和火力楠林为对象,将收集于2017年2月各林分土壤表层(0~20 cm)、中层(20~40 cm)和底层(40~60 cm)的样品进行细菌16S rRNA基因高通量测序,基于该数据分析将马尾松林转换成乡土阔叶林后土壤细菌群落分类与系统发育的多样性和结构的变化特征。[结果]表明:(1)马尾松林改造成米老排林和火力楠林后,显著增加了土壤表层细菌群落分类与系统发育多样性,这主要由土壤含水量(SWC)所致。(2)红锥林、米老排林和火力楠林取代马尾松林后,表层土壤细菌群落分类结构发生了明显变化,而在中层只有红锥林有显著改变,受限于土壤SWC、总磷(TP)、总氮(TN)和碳氮比(C/N)。(3)马尾松林改建为乡土阔叶林后,不同土层的细菌群落系统发育结构仍为聚集,环境过滤始终是调控群落构建的主要因素。(4)在土壤表层和中层,马尾松林转换为红锥林的分类及系统发育转换率高于转换为米老排林和火力楠林,表明红锥林土壤细菌群落稳定性较差。[结论]综上,考虑土壤细菌群落分类和系统发育多样性及转换率,该地将马尾松林改建成米老排林或火力楠林后的土壤细菌群落稳定性高于改建成红锥林后的土壤细菌群落稳定性。[Objective]To explore the characteristics of soil bacterial community diversity and structure with the treatment of conversion of coniferous into broadleaved plantations.[Method]The bacterial 16S rRNA gene high-throughput sequencing was performed on samples of the coniferous Pinus massoniana Lamb plantation(PMP)as well as native broadleaved Castanopsis hystrix Miq.plantation(CHP),Mytilaria lao-sensis Lec.plantation(MLP)and Michelia macclurei Dandy plantation(MMP)in the southern subtropical China collected from the topsoil(0-20 cm)、middle layer(20-40 cm)and subsoil(40-60 cm)under each stand in February 2017.The diversity and structure of soil bacterial community taxonomy and phylogeny after conversion from PMP to native broadleaved plantations were studied.[Results]The results showed that:(1)The taxonomic and phylogenetic diversity of bacterial communities in topsoil increased signific-antly after conversion from PMP to MLP and MMP,which mainly caused by the soil water content(SWC).(2)After CHP,MLP and MMP replaced PMP,the taxonomic structure of topsoil bacterial community changed markedly,and only CHP changed significantly in the middle layer,which were limited by soil SWC,total phosphorus(TP),total nitrogen(TN)and carbon-nitrogen ratio(C/N).(3)After the transforma-tion from PMP to native broadleaved plantations,the phylogenetic structure of bacterial community re-mains clustered in vas soil layers,and environmental filtration was still the main factor regulating com-munity assembly.(4)In the topsoil and middle layer,the taxonomic and phylogenetic turnover rates from PMP to CHP were higher than that from PMP to MLP or MMP,indicating that stability of the soil bacterial community in the CHP stand was poor.[Conclusion]Overall,considering the diversity and turnover rates of soil bacterial community taxonomy and phylogeny,the stability of the soil bacterial community after con-verting PMP into MLP or MMP is higher than that after converting to CHP.

关 键 词：针叶林 乡土阔叶林 土壤细菌群落多样性 系统发育结构 群落构建 

分 类 号：S718.5[农业科学—林学]