基于SSR标记构建江西杉木核心种质及其分子身份证  

Construction of Core Collection and DNA Fingerprinting of Chinese fir Germplasms from Jiangxi based on SSR Markers

作　　者：娄永峰[1] 朱柯帆 宋晓琛[1] 冷春晖 陈兴彬 肖复明[1] LOU Yong-feng;ZHU Ke-fan;SONG Xiao-chen;LENG Chun-hui;CHEN Xing-bin;XIAO Fu-ming(Jiangxi Provincial Key Laboratory of Plant Biotechnology,Jiangxi Academy of Forestry,Nanchang 330032,Jiangxi,China;Xinfeng County Seed Farm,Xinfeng 341602,Jiangxi,China)

机构地区：[1]江西省林业科学院,江西省植物生物技术重点实验室,江西南昌330032 [2]信丰县林木良种场,江西信丰341602

出　　处：《林业科学研究》2023年第6期78-86,共9页Forest Research

基　　金：国家重点研发计划项目任务(NO.2022YFD2200201-04);江西省重点研发计划项目(NO.20223BBF61005);江西省林业科技创新专项(NO.202006、202111、202112)。

摘　　要：[目的]分析江西杉木种质资源的遗传多样性,构建其核心种质;在此基础上,构建核心种质分子身份证,为江西杉木种质的进一步研究与利用提供理论依据和核心材料。[方法]以江西地区的杉木种质为材料,利用SSR标记开展遗传多样性分析,并基于等位基因数最大化原则(M策略)构建核心种质,通过遗传多样性参数及其保留比例进行核心种质评价,结合遗传多样性参数的t检验和主坐标分析(PCoA)验证和确认核心种质的代表性。基于最少标记鉴定最多种质的原则,选择高效SSR标记,构建江西杉木核心种质的SSR分子指纹图谱和分子身份证。[结果]20个SSR标记在495份种质中共检测到122个等位基因数(Na),平均Shan-non’s信息指数(I)、平均Nei’s遗传多样性指数(H)、平均观测杂合度(Ho)和平均期望杂合度(He)分别为0.762、0.400、0.394和0.400,表明江西杉木种质的遗传多样性较丰富。采用M策略构建了含有52份材料的江西杉木核心种质,10.5%的核心种质保留了原种质100.0%的Na、107.4%的有效等位基因数(Ne)、115.1%的I、109.0%的H、104.1%的Ho、110.0%的He和111.2%的PIC;t检验表明以上遗传多样性参数与原种质均没有显著差异,主坐标分析(PCoA)显示核心种质在原种质的主坐标图分布均匀,说明构建的核心种质具有代表性。按多态性信息含量(PIC)值高低顺序,依次增加标记数量,结合UPGMA聚类,发现4个SSR分子标记,H97与H286、CLSSR9、CLSSR37相结合,可将52份江西杉木核心种质鉴别,据此构建了52份核心种质的SSR分子指纹图谱、分子身份证条形码和二维码。[结论]江西杉木种质资源的遗传多样性较丰富,构建的江西52份杉木核心种质具有代表性,选择上诉4个高效SSR标记可有效鉴别核心种质,并成功绘制了江西杉木核心种质的分子身份证。[Objective]To analyze the genetic diversity of the Cunninghamia lanceolata germplasms from Jiangxi Province,and a core collection of C.lanceolata was constructed.The molecular ID of the ger-mplasms was studied for providing theoretical basis and core materials for further research and utilization of the germplasms.[Method]The genetic diversity of the germplasms was analyzed by SSR markers.A core collection was constructed through the allele number maximization strategy(M strategy).The core collection of the germplasms was assessed by the genetic diversity parameters and their retention ratio.The the core collection was confirmed with t-test and PCoA analysis.Based on the principle of distinguish-ing the most varieties using the fewest marker,the more effective SSR markers were selected to construct the molecular fingerprints and IDs of core germplasms.[Result]A total of 122 alleles were detected in the 495 germplasms by 20 SSR markers.The means of Shannon's information index(I),Nei's genetic di-versity index(H),observed heterozygosity(Ho),and expected heterozygosity(He)were 0.762,0.400,0.394,and 0.400,respectively,which indicated a relatively high genetic diversity of the germplasms.The core collection of the 52 germplasms was constructed by M strategy in Core finder software.10.5%of the core collection retained 100.0%Na,107.4%Ne,115.1%I,109.0%H,104.1%Ho,110.0%He and 111.2%PIC of the original collection.The t-test analysis suggested that there were no significant differences between the core collection and original collection.This result was further confirmed by the PCoA analysis.Combining with the 4 SSR markers,H97 and H286,CLSSR9 and CLSSR37,the 52 core collections could be identified.And the molecular fingerprints and IDs of core collections were constructed.The molecular IDs were illustrated as barcodes and QR codes.[Conclusion]There is relatively abundant genetic di-versity of C.lanceolata germplasms in Jiangxi.The 52 core collections are representative.The 4 more ef-fective SSR markers mentioned above can i

关 键 词：杉木 遗传多样性 核心种质 分子身份证 SSR标记 

分 类 号：S791.27[农业科学—林木遗传育种]