郭岩山自然保护区丝栗栲天然林分土壤微生物群落沿海拔梯度变化  

Changes of Soil Microbial Community along the Altitude Gradient in the Natural Stand of Castanopsis fargesii in the Guoyan Mountain

作　　者：汪运祥 吴航湦 何冬梅 苏仪 王子杨 潘龙 廖晓丽[3] 靳少非 郑德祥[1] WANG Yun-xiang;WU Hang-sheng;HE Dong-mei;SU Yi;WANG Zi-yang;PAN Long;LIAO Xiao-li;JIN Shao-fei;ZHENG De-xiang(College of Forestry,Fujian Agricultural and Forestry University,Fuzhou 350002,Fujian,China;State-owned Forest Farm of Shunchang Guandun,Shunchang 353200,Fujian,China;College of Geography and Oceanography,Minjiang University,Fuzhou 350108,Fujian,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]顺昌县官墩国有林场,福建顺昌353200 [3]闽江学院地理与海洋学院,福建福州350108

出　　处：《林业科学研究》2023年第6期134-143,共10页Forest Research

基　　金：国家自然科学基金面上项目(32071760);福建农林大学生态文明建设与管理交叉学科建设项目(71202103E);福建省自然科学基金面上项目(2023J01436)。

摘　　要：[目的]沿着海拔梯度探究郭岩山丝栗栲天然林分土壤微生物多样性和群落组成变化及其与土壤性质的相关关系,揭示气候变化对中国中亚热带中低海拔小跨度梯度下丝栗栲土壤微生物多样性和群落组成变化规律及其影响因素。[方法]选取郭岩山自然保护区海拔500~900 m梯度下丝栗栲天然林分的表层土壤,测定其理化性质,并进行Illumina高通量测序,采用相关性分析和冗余分析等方法研究土壤微生物多样性和群落组成特征及其影响因素。[结果]土壤细菌α多样性并没有呈现明显的海拔分布格局,而真菌的α多样性沿海拔梯度先下降后上升,这与大跨度海拔梯度的研究存在差异;酸杆菌门(Acidobacteria)、放线菌门(Actinobac-teria)和变形菌门(Proteobacteria)是细菌群落的3个主要菌门,norank_f_norank_o_subgroup_2、nor-ank_f_xanthobacteraceae是细菌群落的两个主要菌属;担子菌门(Basidiomycota)、子囊菌门(Ascomyco-ta)是真菌群落的两个主要菌门,Russula、Lactifluus是真菌群落的两个主要菌属,说明了小跨度的海拔梯度不会显著影响优势微生物菌门和属的变化;优势细菌门及属相对丰度及群落组成与土壤pH值、全磷和碱解氮密切相关,而土壤全磷和速效钾是影响真菌群落组成和优势门及属相对丰度的最重要因素,土壤性质可以很好地预测土壤微生物群落的变化,即使在小跨度海拔梯度上亦是如此。[结论]土壤性质和海拔高度共同影响了郭岩山自然保护区土壤微生物的生态地理学分布。本研究将为南方亚热带常绿阔叶林小跨度海拔梯度土壤微生物多样性研究和群落组成保护提供科学依据。[Objective]To investigate the changes of soil microbial community along the altitude gradient in the natural stand of Castanopsis fargesii and their correlation with soil physicochemical properties in the Guoyan Mountain for revealing the impact of climate change on soil microbial diversity and community composition and the influencing factors of Castanopsis fargesii under the small-scale elevational gradients in the subtropical region of China.[Methods]The topsoils of the natural stand of Castanopsis fargesii along an elevation gradient in the Guoyan Mountain Nature Reserve were selected and their physicochem-ical properties were determined,and Illumina high-throughput sequencing was performed.The correlation analysis and redundancy analysis were used to study the characteristics of soil microbial diversity and community composition and their influencing factors.[Results]The alpha diversity of soil bacteria did not show a clear elevational pattern,while the alpha diversity of fungi first decreased and then increased along the elevation gradient,which was different from the studies across large-scale elevational gradients.The dominant bacterial phyla were Acidobacteria,Actinobacteria and Proteobacteria;the dominant bacterial genera were norank_f_norank_o_subgroup_2 and norank_f_xanthobacteraceae;the dominant fungal phyla were Basidiomycota and Ascomycota;the dominant fungal genera were Russula and Lactifluus.Our results showed that small-scale elevational gradients did not significantly affect the changes in the domin-ant microbial phyla and genera.The relative abundance and community composition of the dominant bac-terial phyla and genera were closely related to soil pH,total phosphorous and available nitrogen,while soil total phosphorus and available potassium were the most important factors affecting the composition of fungal communities.Soil properties could well predict the changes in soil microbial communities,even on small scale elevational gradients.[Conclusion]Soil properties and altitude both influence

关 键 词：自然保护区 丝栗栲 土壤微生物 海拔梯度 

分 类 号：S714[农业科学—林学]