Pre-mating nitenpyram exposure in male mice leads to depression-like behavior in offspring by affecting tryptophan metabolism in gut microbiota  

作　　者：Sen Yan Wei Sun Sinuo Tian Zhiyuan Meng Jinling Diao Zhiqiang Zhou Li Li Wentao Zhu 

机构地区：[1]Innovation Center of Pesticide Research,Department of Applied Chemistry,College of Science,China Agricultural University,Beijing 100193,China [2]College of Plant Protection/Joint International Research Laboratory of Agriculture&Agri-Product Safety,Yangzhou University,Yangzhou 225009,China [3]College of Plant Protection,Shanxi Agricultural University,Taiyuan 030031,China

出　　处：《Journal of Environmental Sciences》2024年第3期120-130,共11页环境科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (No.2016YFD0200202);the 2115 Talent Development Program of China Agricultural University;the Fundamental Research Program of Shanxi Province (No.20210302124131)。

摘　　要：Several studies have confirmed that the health status of the paternal affects the health of the offspring,however,it remains unknown whether paternal exposure to pesticides affect the offspring health.Here,we used untargeted metabolomics and 16S rRNA sequencing technology,combined with tail suspension test and RT-qPCR to explore the effects of paternal exposure to nitenpyram on the neurotoxicity of offspring.Our results found that the paternal exposure to nitenpyram led to the offspring’s depressive-like behaviors,accompanied by the reduction of tryptophan content and the disorder of microbial abundance in the gut of the offspring.Further,we determined the expression of tryptophan metabolism-related genes tryptophanase(tnaA)and tryptophan hydroxylase 1(TpH1)in gut bacteria and colonic tissues.We found that tryptophan is metabolized to indoles rather than being absorbed into colonocytes,which coursed the reduce of tryptophan availability after nitenpyram exposure.In conclusion,our study deepens our understanding of the intergenerational toxic effects of pesticides.

关 键 词：NITENPYRAM Depressive-like behaviors Gut microbiota Tryptophan metabolism 

分 类 号：X503[环境科学与工程—环境工程]