Enrichment of anammox biomass during mainstream wastewater treatment driven by achievement of partial denitrification through the addition of bio-carriers  

作　　者：Yuqing Ma Bo Wang Xiaodi Li Shuo Wang Wen Wang Yongzhen Peng 

机构地区：[1]National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Engineering Research Center of Beijing,Beijing University of Technology,Beijing 100124,China

出　　处：《Journal of Environmental Sciences》2024年第3期181-194,共14页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.22176007),the Beijing Natural Science Foundation (No.8212022);the fund of innovation and entrepreneurship leading team project in Guangzhou (No.CYLJTD-201607);the National Key Research and Development Program of China (No.2021YFC3200605)。

摘　　要：Anammox is widely considered as the most cost-effective and sustainable process for nitrogen removal.However,how to achieve the enrichment of anammox biomass remains a challenge for its large-scale application,especially in mainstream wastewater treatment.In this study,the feasibility of enrichment of anammox biomass was explored through the realization of partial denitrification and the addition of bio-carriers.By using ordinary activated sludge,a sequencing batch reactor(SBR)followed by an up-fow anaerobic sludge bed(UASB)was operated at 25±2℃ for 214 days.The long-term operation was divided into five phases,in which SBR and UASB were started-up in Phases I and II,respectively.By eliminating oxygen and adjusting the infow ratios in Phases III-V,advanced nitrogen removal was achieved with the effuent total nitrogen being 4.7 mg/L and the nitrogen removal efficiency being 90.5%in Phase V.Both in-situ and ex-situ activity tests demonstrated the occurrence of partial denitrification and anammox.Moreover,16S rRNA high-throughput sequencing analysis revealed that Candidatus Brocadia was enriched from below the detection limit to in biofilms(0.4%in SBR,2.2%in UASB)and the foc sludge(0.2%in SBR,1.3%in UASB),while Thauera was mainly detected in the foc sludge(8.1%in SBR,8.8%in UASB),which might play a key role in partial denitrification.Overall,this study provides a novel strategy to enrich anammox biomass driven by rapid achievement of partial denitrification through the addition of bio-carriers,which will improve large-scale application of anammox processes in mainstream wastewater treatment.

关 键 词：ENRICHMENT Anammox biomass Bio-carriers Partial denitrification Mainstream wastewater 

分 类 号：X703[环境科学与工程—环境工程]