Unveiling the performance of a novel alkalizing bacterium Enterobacter sp.LYX-2 in immobilization of available Cd  

作　　者：Yixin Luo Min Liao Xiongxiong Lu Na Xu Xiaomei Xie Weiming Gao 

机构地区：[1]College of Environmental and Resource Science,Zhejiang University,Hangzhou 310058,China [2]Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment,Hangzhou 310058,China [3]National Demonstration Center for Experimental Environmental and Resources Education,Zhejiang University,Hangzhou 310058,China

出　　处：《Journal of Environmental Sciences》2024年第3期245-257,共13页环境科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (No.2018YFC1800403);the National Natural Science Foundation of China (No.41571226);the Scientific Research Fund of Zhejiang University (No.XY2021020)。

摘　　要：A novel alkalizing strain Enterobacter sp.LYX-2 that could resist 400 mg/L Cd was isolated from Cd-contaminated soil,which immobilized 96.05%Cd^(2+)from medium.Cd distribution analysis demonstrated that more than half of the Cd^(2+)was converted into extracellular precipitated Cd through mobilization of the alkali-producing mechanism by the strain LYX-2,achieving the high immobilization efficiency of Cd^(2+).Biosorption experiments revealed that strain LYX-2 had superior biosorption capacity of 48.28 mg/g for Cd.Pot experiments with Brassica rapa L.were performed with and without strain LYX-2.Compared to control,15.92%bioavailable Cd was converted to non-bioavailable Cd and Cd content in aboveground vegetables was decreased by 37.10%with addition of strain LYX-2.Available Cd was mainly immobilized through extracellular precipitation,cell-surface biosorption and intracellular accumulation of strain LYX-2,which was investigated through Cd distribution,Scanning Electron Microscope and Energy-Dispersive X-ray Spectroscopy(SEM-EDS),Fourier Transform Infrared Spectroscopy(FT-IR),X-ray Photoelectron Spectroscopy(XPS)and Transmission Electron Microscopy(TEM)analysis.In addition,the application of strain LYX-2 significantly promoted the growth of vegetables about 2.4-fold.Above results indicated that highly Cd-resistant alkalizing strain LYX-2,as a novel microbial passivator,had excellent ability and reuse value to achieve the remediation of Cd-contaminated soil coupled with safe production of vegetables simultaneously.

关 键 词：CADMIUM Enterobacter sp.LYX-2 Cd Immobilization Microbial passivator 

分 类 号：X53[环境科学与工程—环境工程] X172