Perturbation of autophagy pathways in murine alveolar macrophage by 2D TMDCs is chalcogen-dependent  

作　　者：Xiaofei Zhou Weitao Jin Rui Zhang Xuan Mao Jianbo Jia Hongyu Zhou 

机构地区：[1]College of Science&Technology,Hebei Agricultural University,Huanghua 061100,China [2]Institute of Environmental Research at Greater Bay Area,Key Laboratory for Water Quality and Conservation of the Pearl River Delta,Ministry of Education,Guangzhou University,Guangzhou 510006,China

出　　处：《Journal of Environmental Sciences》2024年第1期97-107,共11页环境科学学报（英文版）

基　　金：supported by the Special Scientific Research Fund for Talents Introduced of Hebei Agricultural University (No.YJ2019030);the National Natural Science Foundation of China (Nos.22276042,21906035);the Pearl River Young Talents Program of Guangdong Province (No.2017GC010269)。

摘　　要：Increasing risks of incidental and occupational exposures to two-dimensional transition metal dichalcogenides(2D TMDCs)due to their broad application in various areas raised their public health concerns.While the composition-dependent cytotoxicity of 2D TMDCs has been well-recognized,how the outer chalcogenide atoms and inner transition metal atoms differentially contribute to their perturbation on cell homeostasis at non-lethal doses remains to be identified.In the present work,we compared the autophagy induction and related mechanisms in response to WS_(2),NbS_(2),WSe_(2)and Nb Se_(2)nanosheets exposures in MH-S murine alveolar macrophages.All these 2D TMDCs had comparable physicochemical properties,overall cytotoxicity and capability in triggering autophagy in MH-S cells,but showed outer chalcogen-dependent subcellular localization and activation of autophagy pathways.Specifically,WS_(2)and NbS_(2)nanosheets adhered on the cell surface and internalized in the lysosomes,and triggered m TOR-dependent activation of autophagy.Meanwhile,WSe_(2)and Nb Se_(2)nanosheets had extensive distribution in cytoplasm of MH-S cells and induced autophagy in an m TOR-independent manner.Furthermore,the 2D TMDCs-induced perturbation on autophagy aggravated the cytotoxicity of respirable benzo[a]pyrene.These findings provide a deeper insight into the potential health risk of environmental 2D TMDCs from the perspective of homeostasis perturbation.

关 键 词：Chalcogen group Autophagy pathway Cell function Endosomal escape Inhalation toxicity 

分 类 号：X503[环境科学与工程—环境工程]