茸毛小长蝽的线粒体基因组测序和分析  

Mitochondrial genome and phylogenetic analysis of Nysius graminicola(Kolenati,1845)

作　　者：林兴雨 宋南[1] LIN Xingyu;SONG Nan(College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学植物保护学院,河南郑州450046

出　　处：《浙江农业学报》2023年第12期2878-2889,共12页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(U1904104)。

摘　　要：为探究茸毛小长蝽Nysius graminicola(Kolenati,1845)的线粒体基因组结构及长蝽总科的系统发育关系,利用二代测序方法获得了茸毛小长蝽线粒体全基因组序列。在系统发育分析中,使用长蝽总科17个科52个物种作为内群,并选择缘蝽总科Coreoidea的2个物种作为外群,利用最大似然法和贝叶斯法重建长蝽总科的系统发育关系。茸毛小长蝽的线粒体基因组包含37个基因(13个蛋白质编码基因、22个转运RNA基因和2个核糖体RNA基因)和1个非编码控制区,全长为16760 bp(GenBank登录号:OQ553818)。茸毛小长蝽线粒体基因组的13个蛋白质编码基因的起始密码子除nad2利用GTG和cox 1利用TTG作为起始,其余蛋白质编码基因都是以ATN开头,终止密码子除cox1、cox2、nad 3、cob和nad 1以不完整的T作为结尾,其余蛋白质编码基因都是以TAA结尾。除trnS 1因缺少DHU臂而形成一个简单的环,无法形成稳定的三叶草结构外,其余转运RNA基因均能形成典型的三叶草结构。两个方法构建的系统发育树的结果均表明:长蝽科和地长蝽科为非单系群,梭长蝽科、室翅长蝽科、跷蝽科、杆长蝽科和束长蝽科为单系群。此外,茸毛小长蝽与N.fuscovittatus有较近的亲缘关系。To explore the mitochondrial genome of Nysius graminicola(Kolenati,1845)and the phylogeny of Lygaeoidea,we newly sequenced the complete mitochondrial genome of N.graminicola by using the next-generation sequencing method.In phylogenetic analyses,we selected 17 family including 52 exemplars of Lygaeoidea as ingroups.Two species of Coreoidea was used as outgroup.We reconstructed the phylogenetic relationships using Maximum likelihood and Bayesian inference methods.The sequenced genome is a circular molecule of 16760 bp in length(Genbank accession No.:OQ553818)which possesses 13 protein-coding genes,22 transfer RNA genes,2 ribosomal RNA genes,and putative control regions as well.For most of the protein-coding genes the star codons were ATN except for nad2 using GTG and cox1 using TTG as star codon.Most of the protein-coding genes terminated with the stop codon TAA,while cox1,cox2,nad3,cob and nad1 employed the incomplete T as the stop codon.All tRNA genes can be folded into a typical cloverleaf structure,with the except of the trnS 1-AGN.The trnS1 lacks the DHU arm.Both phylogenetic inference methods showed largely identical tree topological structures.The families of Lygaeidae and Rhyparochromidae were non-monophyletic.However,the families of Pachygronthidae,Heterogastridae,Berytidae,Blissidae and Malcidae were monophyletic.In addition,N.graminicola is closely related to N.fuscovittatus.

关 键 词：半翅目 长蝽总科 茸毛小长蝽 线粒体基因组 系统发育 

分 类 号：Q963[生物学—昆虫学]