稻虾种养系统水稻根际土壤活性有机碳、微生物群落结构及其相互关系  

Active organic carbon,microbial community structure and their relationship in rice rhizosphere soil of rice-crayfish co-culture systems

作　　者：郝柳柳 代梨梨 彭亮[2] 陈思媛 陶玲[2] 李谷[2] 张辉[2] HAO Liuliu;DAI Lili;PENG Liang;CHEN Siyuan;TAO Ling;LI Gu;ZHANG Hui(College of Life Science,Huzhou University,Huzhou 313000,Zhejiang,China;Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 420322,China)

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000 [2]中国水产科学研究院长江水产研究所,湖北武汉420322

出　　处：《浙江农业学报》2023年第12期2901-2913,共13页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(U20A2010,31902378);国家大宗淡水鱼产业技术体系(CARS-45-22)。

摘　　要：为了探明稻虾种养系统土壤活性有机碳的动态转化规律和潜在机制,基于稻田根际土壤有机碳的测定和16S rDNA扩增子高通量测序技术,对比研究了水稻单作(MR)和稻虾种养(CR)两种模式在水稻分蘖期、抽穗期和成熟期根际土壤活性有机碳含量和微生物群落的变化规律,并分析了它们之间的相互关系。结果表明,相比MR模式,CR模式在分蘖期、抽穗期和成熟期水稻根际土壤总有机碳(TOC)和颗粒有机碳(POC)含量(P<0.05)显著升高,抽穗期、成熟期易氧化有机碳(LOC)含量,以及分蘖期微生物量碳(MBC)含量显著增加,但抽穗期和成熟期可溶性有机碳(DOC)含量显著降低。主成分分析表明,CR模式下的微生物群落与MR模式存在明显差异。CR模式抽穗期unclassified_Anaerolineaceae,以及分蘖期和抽穗期unclassified_Steroidobacteraceae的相对丰度较MR模式分别显著增加了93.53%、120.85%和389.79%。代谢通路分析表明,CR模式水稻分蘖期原核生物的碳固定途径、甲烷代谢等代谢通路的相对丰度显著高于MR模式。相关性分析和冗余分析的结果表明,根际各活性有机碳含量与微生物群落组成存在显著相关性,POC可能是影响稻虾系统水稻根际土壤微生物群落的重要因素。In order to investigate the dynamic transformation of soil active organic carbon and the potential mechanism in rice-crayfish co-culture systems,the variations of soil active organic carbon contents and microbial communities in rice rhizosphere were examined with the rice monoculture(MR)and rice-crayfish co-culture(CR)systems,and samples were collected at the tillering stage,heading stage and maturity stage of rice.It was shown that compared with the MR system,the contents of total organic carbon and particulate organic carbon in rice rhizosphere soil were significantly(P<0.05)increased in the CR system at the tillering stage,heading stage and maturity stage of rice,and the contents of labile organic carbon at the heading stage and the maturity stage and microbial biomass carbon at the tillering stage were also significantly increased,but the contents of dissolved organic carbon at the heading stage and the maturity stage stage were significantly decreased in the CR system.Principle component analysis results showed that the microbial communities in the CR system were different from those in the MR system.The abundances of unclassified_Anaerolineaceae at the heading stage and unclassified_Steroidobacteraceae at the tillering stage and the heading stage in the CR systems were sginificantly increased by 93.53%,120.85% and 389.79%,respectively,compared with the MR system.Metabolism pathways analysis result indicated that the pathways such as carbon fixation pathways in prokaryotesand and methane metabolism in the CR systems were significantly higher than those in the MR systems.Correlation analysis and redundancy analysis results showed that there were significant correlations within rhizosphere active organic carbon contents and microbial community composition,among which,the particulate organic carbon content might have an important effect on the microbial composition.

关 键 词：稻虾种养模式 水稻生育期 根际有机碳 功能预测 

分 类 号：S153[农业科学—土壤学] S345[农业科学—农业基础科学]