lncRNA1926和lncRNA3223在肉鸡胚胎肝细胞脂肪代谢中的功能研究  

Functional study on lncRNA1926 and lncRNA3223 in lipid metabolism of broiler embryonic primary hepatocytes

作　　者：李辉 张惠莉[1,2,3] 王有栋 程博涵 冷丽 栾鹏[1,2,3] 李玉茂 白雪[1,2,3] LI Hui;ZHANG Huili;WANG Youdong;CHENG Bohan;LENG Li;LUAN Peng;LI Yumao;BAI Xue(Key Laboratory of Chicken Genetics and Breeding,Ministry of Agriculture and Rural Affairs,Harbin 150030,China;Key Laboratory of Animal Genetics,Breeding and Reproduction,Education Department of Heilongjiang Province,Harbin 150030,China;School of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]农业农村部鸡遗传育种重点实验室,哈尔滨150030 [2]黑龙江省普通高等学校动物遗传育种与繁殖重点实验室,哈尔滨150030 [3]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《东北农业大学学报》2023年第8期17-26,共10页Journal of Northeast Agricultural University

基　　金：黑龙江省博士后面上项目(LBH-Z20111);国家肉鸡产业技术体系项目(CARS-41)。

摘　　要：RNA-Seq分析发现LOC107051926(命名为lncRNA1926)和LOC112533223(命名为lncRNA3223)在肉鸡胚胎肝脏组织脂肪代谢过程发挥重要作用,但其具体功能尚不清楚。采用油红O染色、GPO-PAP和RT-qPCR方法对lncRNA1926和lncRNA3223作功能研究。结果表明,敲低lncRNA1926或lncRNA3223后,显著降低胚胎原代肝细胞中脂滴沉积和甘油三酯含量;lncRNA1926是脂肪酸合成(ACC、FAS)、甘油三酯合成(GPAT)、脂肪酸降解(CPT1)相关基因正调控因子;lncRNA3223是脂肪酸合成(ACC、FAS)、甘油三酯合成(GPAT、DGAT和MOGAT2)相关基因正调控因子。敲低lncRNA1926或lncRNA3233,通过抑制脂肪酸合成和甘油三酯合成相关基因表达降低甘油三酯含量,从而降低胚胎原代肝细胞中脂滴沉积。研究结果有助于增进对鸡肝脏脂肪代谢分子调控的理解,为肉鸡体脂性状遗传改良提供理论依据。RNA-Seq analysis shows that LOC107051926(named lncRNA1926)and LOC112533-223(named lncRNA3223)play important roles in fat metabolism in broiler embryo liver tissues,but their specific functions remain unclear.In the present study,oil red O staining,GPO-PAP and RT-qPCR methods were used to verify the functions of lncRNA1926 and lncRNA3223 in fat metabolism.The results showed that knockdown of lncRNA1926 or lncRNA3223 significantly reduced lipid deposition and triglyceride(TG)content in embryonic primary hepatocytes.lncRNA1926 was a positive regulator of genes involved in fatty acid synthesis(ACC and FAS),TG synthesis(GPAT)and fatty acid degradation(CPT1);and lncRNA3223 was a positive regulator of genes involved in fatty acid synthesis(ACC and FAS)and TG synthesis(GPAT,DGAT and MOGAT2).Knockdown of lncRNA1926 or lncRNA3233 reduced TG content by inhibiting the expression of genes involved in fatty acid synthesis and TG synthesis,thus reducing lipid deposition in embryonic primary hepatocytes.These results were helpful to improve the understanding of molecular regulation of fat metabolism in chicken liver and provide theoretical basis for genetic improvement of broiler abdominal fat traits.

关 键 词：肉鸡 lncRNA 脂肪代谢 胚胎原代肝细胞 

分 类 号：S831.2[农业科学—畜牧学]