不同抗性柑橘对野生型和柑橘链格孢毒素合成受阻褐斑病菌侵染的转录响应差异  

Differential transcriptome analysis of different resistant citrus varieties infected with wild-type and toxin synthesis-impaired strains causing Alternaria brown spot

作　　者：马慧 马海杰 焦晨 李红叶[1] MA Hui;MA Haijie;JIAO Chen;LI Hongye(Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects/Zhejiang Provincial Key Laboratory of Biology of Crop Pathogens and Insects/College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang,China;Ministry of Agriculture and Rural Affairs Key Laboratory of Quality and Safety Control of Subtropical Fruits and Vegetables/College of Horticulture Science,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]农业农村部作物病虫分子生物学重点实验室/浙江省作物病虫生物学重点实验室/浙江大学农业与生物技术学院,浙江杭州310058 [2]农业农村部亚热带果品蔬菜质量安全控制重点实验室/浙江农林大学园艺科学学院,浙江杭州311300

出　　处：《浙江大学学报（农业与生命科学版）》2023年第6期787-801,812,共16页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家现代农业(柑橘)产业技术体系建设专项(CARS-26);国家自然科学基金项目(32102148);浙江省重点研发计划项目(2019C02022)。

摘　　要：为探讨不同抗性柑橘在与褐斑病菌互作过程中对柑橘链格孢毒素的响应差异,以抗病品种克里曼丁(Citrus clementina Hort.ex Tan.cv.Clementine)和感病品种丹西红橘(Citrus reticulata Blanco cv.Dancy)为材料,分别接种野生型产毒菌株Z7和毒素合成基因ACTT6双敲除突变体菌株ΔΔACTT6,并利用转录组测序技术对接种24 h和48 h后的叶片进行基因表达分析。比较2种柑橘对产毒和不产毒菌株侵染后的转录差异发现,2个品种的先天免疫通路(病原物相关分子模式触发的免疫反应和效应子触发的免疫反应)、病程相关蛋白、WRKY转录因子、次生代谢途径等基因均受产毒菌株诱导表达。在接种后期(48 h),产毒菌株主要激活感病品种的脂质和蛋白质等大分子降解过程,说明其细胞完整性因毒素产生而遭到破坏;而抗病柑橘则主要富集解毒和茉莉酸代谢等生物过程,其中特异激活的多药及毒素化合物外排蛋白(multidrug and toxic compound extrusion protein,MATE)基因与其抵御褐斑病菌毒素的能力有关。本研究初步明确了柑橘对褐斑病菌毒素响应的分子调控机制和关键功能基因,可为柑橘抗褐斑病菌分子育种提供理论依据。To investigate the differences in the response of citrus varieties with different resistances to Alternaria citri toxin(ACT)during interaction with the Alternaria alternata pathotype tangerine causing Alternaria brown spot,we inoculated the wild-type toxin-producing strain Z7 and the ACT synthesis-impaired mutant strainΔΔACTT6 onto the leaves of resistant variety Clementine(Citrus clementina Hort.ex Tan.cv.Clementine)and the susceptible variety Dancy(Citrus reticulata Blanco cv.Dancy),and analyzed the gene expressions at 24 h and 48 h after inoculation using transcriptome sequencing technology.By comparing the corresponding differences in transcription between the two citrus varieties against the toxin-producing and non-toxin-producing strains,we found that the genes involved in the pathogen-associated molecular patterns(PAMPs)-triggered immunity(PTI)and effector-triggered immunity(ETI)pathways,pathogenesis-related proteins,WRKY transcription factors,and the secondary metabolic pathway were induced by the toxin-producing strain in both the varieties.At the later stage of inoculation(48 h),the toxin-producing strain mainly activated macromolecular degradation processes such as lipid and protein degradation in the susceptible citrus variety,indicating that its cellular integrity was disrupted due to toxin production,whereas the resistant citrus variety was mainly enriched in biological processes such as detoxification and jasmonic acid metabolism.Some multidrug and toxic compound extrusion protein(MATE)genes were specifically activated in the resistant citrus variety,which implies their ability to resist fungal toxins.In conclusion,the molecular regulatory mechanisms and key functional genes involved in the response of citrus varieties to ACT are preliminarily clarified,which provide a theoretical basis for the molecular breeding of citruses against Alternaria brown spot.

关 键 词：柑橘褐斑病 链格孢菌橘致病型 柑橘链格孢毒素 转录组 

分 类 号：S432.1[农业科学—植物病理学]