广州地区无偿献血人群不同时期新冠病毒抗体检测分析  

Detection and analysis of antibodies against SARS-CoV-2 in blood donors at different periods in Guangzhou

作　　者：单振刚[1] 羊华龙 杨乐东[1] 黄杰庭[1] 王敏[1] 许茹[1] 廖峭[1] 钟惠珊 刘博超 付涌水[1,2] 戎霞 SHAN Zhengang;YANG Hualong;YANG Ledong;HUANG Jieting;WANG Min;XU Ru;LIAO Qiao;ZHONG Huishan;LIU Bochao;FU Yongshui;RONG Xia(Institute of Clinical Blood Transfusion,Guangzhou Blood Center,Guangzhou 510095,China;School of Laboratory Medicine and Biotechnology,Southern Medical University)

机构地区：[1]广州血液中心,广东广州510095 [2]南方医科大学南方医院

出　　处：《中国输血杂志》2023年第12期1094-1097,共4页Chinese Journal of Blood Transfusion

基　　金：广州市科技计划(2023A03J1000)。

摘　　要：目的了解广州市无偿献血人群体内新冠病毒抗体水平,为恢复期血浆采集和临床使用提供数据支持。方法使用酶联免疫吸附法对不同时期在广州血液中心献血的合格献血者血液标本做新冠病毒抗体检测,其中对2023年2月献血的326人检测IgG抗体,444人检测中和抗体;对2023年7月献血的398人同时检测IgG和IgM抗体。结果724份血液标本经生理盐水160倍稀释后有399份的IgG检测结果为反应性(55.11%)。不同性别,不同年龄段的IgG反应性率无差异(P<0.05),但不同时间采集的标本的IgG反应性率有差异(2月25.46%vs 7月79.40%,χ2=210.74,P<0.05,95%CI:7.97,15.98)。398份血液标本经检测有5份为IgM反应性(1.26%)。该5名献血者的IgG检测结果均为反应性,血液核酸检测结果均为阴性。444份血液标本中有440份(99.10%)检出中和抗体,其中71.59%的中和抗体反应性献血者体内的中和抗体水平≥10μg/mL。结论广州地区无偿献血人群有较高的新冠病毒抗体水平,具备为临床提供恢复期血浆的条件。Objective To detect the anti-SARS-CoV-2 antibody levels in blood donors in Guangzhou,so as to provide laboratory data support for the collection and clinical use of convalescent plasma.Methods Anti-SARS-CoV-2 antibodies were measured by ELISA in qualified donors.Among them,326 donors who gave blood in February 2023 were tested for IgG antibodies,444 donors were tested for neutralizing antibodies.In July 2023,398 donors were tested for IgG and IgM.Results 399 of 724 blood samples diluted with normal saline(1∶160)were IgG reactive,with a reactive rate of 55.11%.Chi-square test showed that there was a significant difference in the reactive rate of IgG among samples collected at different times(25.46%in February vs 79.40%in July,χ2=210.74,P<0.01,95%CI:7.97,15.98),but there was no significant difference in the reactive rate between different genders and different age groups.IgM was detected in 5 of 398 blood samples,with a reactive rate of 1.26%.The IgG test results of these five blood donors were all reactive,whereas the nucleic acid test results were negative.Neutralizing antibody was detected in 440 of 444 blood samples,with a reactive rate of 99.10%,and 71.59%of the reactive donors had a neutralizing antibody level of 10μg/mL or more.Conclusion Blood donors in Guangzhou have a high level of SARS-CoV-2 antibody,which is sufficient to provide convalescent plasma for clinical treatment.

关 键 词：献血人群 新冠病毒 抗体 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学]