右美托咪啶对异氟烷诱导大鼠PC12细胞线粒体氧化损伤的影响  

Effect of Dexmedetomidine on Isoflurane-induced Mitochondrial Oxidative Damage in Rat PC12 Cells

作　　者：关佳佳 刘萌萌 于志勇 于权麟 王思爽 李继文 迟良[1] 刘焕奇[1] GUAN Jia-jia;LIU Meng-meng;YU Zhi-yong;YU Quan-lin;WANG Si-shuang;LI Ji-wen;CHI Liang;LIU Huan-qi(College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;Qingdao Smart Village Development Service Center,Qingdao 266100,China)

机构地区：[1]青岛农业大学动物医学院,山东青岛266109 [2]青岛市智慧乡村发展服务中心,山东青岛266100

出　　处：《中国兽医杂志》2023年第12期26-33,共8页Chinese Journal of Veterinary Medicine

基　　金：山东省现代农业产业技术体系牛产业创新团队资助项目(SDAIT-09-05)。

摘　　要：为了分析右美托咪啶对异氟烷致PC12细胞线粒体氧化损伤的保护作用和机制,本试验设立对照(Control)组、异氟烷(ISO)组、右美托咪啶(DEX)组和右美托咪啶加异氟烷(DEX+ISO)组,使用活性氧(ROS)、丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)和超氧化物歧化酶(SOD)检测试剂盒检测细胞氧化应激相关指标;线粒体膜电位试剂盒检测线粒体膜电位,ELISA试剂盒检测细胞色素C(Cyt-C)浓度,TUNEL试剂盒检测细胞凋亡,利用Fura-2 AM荧光探针检测细胞内钙离子(Ca^(2+))浓度。结果显示,与Control组相比,ISO组PC12细胞ROS水平和MDA含量极显著上调(P<0.01),CAT、GPX和SOD活性极显著降低(P<0.01);而与ISO组相比,DEX+ISO组PC12细胞ROS水平极显著降低(P<0.01),MDA含量显著降低(P<0.05),CAT和SOD活性极显著升高(P<0.01),GPX活性显著升高(P<0.05);与Control组相比,ISO组线粒体膜电位极显著下降(P<0.01),而与ISO组相比,DEX+ISO组线粒体膜电位显著上升(P<0.05);与Control组相比,ISO组Cyt-C浓度极显著升高(P<0.01),细胞凋亡率极显著升高(P<0.01),而与ISO组相比,DEX+ISO组Cyt-C浓度显著降低(P<0.05),细胞凋亡率极显著降低(P<0.01);与Control组相比,ISO组Ca 2+浓度极显著升高(P<0.01),而与ISO组相比,DEX+ISO组Ca 2+浓度显著降低(P<0.05)。结果表明,右美托咪啶对异氟烷致PC12细胞氧化应激和线粒体功能障碍有保护作用。In order to analyze the protective effect and mechanism of dexmedetomidine on isoflurane-induced mitochondrial oxidative damage in PC12 cells,this study established control(Control)group,isoflurane(ISO)group,dexmedetomidine(DEX)group,and dexmedetomidine plus isoflurane(DEX+ISO)group.Reactive oxygen species(ROS),malondialdehyde(MDA),catalase(CAT),glutathione peroxidase(GPX),and superoxide dismutase(SOD)assay kits were used to detect cellular oxidative stress-related indicators.The mitochondrial membrane potential assay kit was used to measure mitochondrial membrane potential,the enzyme-linked immunosorbent assay(ELISA)kit was used to measure cytochrome C(Cyt-C)concentration,the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)assay kit was used to detect cell apoptosis,and the Fura-2 AM fluorescent probe was used to measure intracellular calcium ion(Ca 2+)concentration.The results showed that compared to the Control group,the ROS level and MDA content in PC12 cells of the ISO group were significantly upregulated(P<0.01),while the activities of CAT,GPX,and SOD were significantly reduced(P<0.01);compared to the ISO group,the ROS level in PC12 cells of the DEX+ISO group was significantly reduced(P<0.01),MDA content was significantly reduced(P<0.05),and the activities of CAT and SOD were significantly increased(P<0.01),while GPX activity was significantly increased(P<0.05);compared to the Control group,the mitochondrial membrane potential in the ISO group significantly decreased(P<0.01),while compared to the ISO group,the mitochondrial membrane potential in the DEX+ISO group significantly increased(P<0.05);compared to the Control group,the Cyt-C concentration in the ISO group significantly increased(P<0.01),and the cell apoptosis rate significantly increased(P<0.01);compared to the ISO group,the Cyt-C concentration in the DEX+ISO group significantly decreased(P<0.05),and the cell apoptosis rate significantly decreased(P<0.01);compared to the Control group,the Ca 2+concentration in the ISO gr

关 键 词：右美托咪啶 异氟烷 氧化应激 线粒体功能障碍 

分 类 号：S854.4[农业科学—临床兽医学]