Paeonilactone B调节巨噬细胞发挥抗炎活性的作用机理研究  

Anti-inflammatory Activity and Mechanism of Paeonilactone B by Regulating Macrophage

作　　者：廖彩岑 宿露 杨琼惠 范韦佟 李洪梅[1] 李蓉涛[1] 刘丹[1] LIAO Caicen;SU Lu;YANG Qionghui;FAN Weitong;LI Hongmei;LI Rongtao;LIU Dan(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《昆明理工大学学报（自然科学版）》2023年第6期88-96,共9页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：国家自然科学基金项目(82204248).

摘　　要：研究了中药赤芍中的单萜类化合物paeonilactone B的体外抗炎活性及其作用机制.通过脂多糖(LPS)诱导RAW264.7单核巨噬细胞建立体外抗炎模型,发现paeonilactone B能显著抑制LPS诱导的巨噬细胞中NO的产生,IC 50值为6.09±1.81μmol/L.对paeonilactone B进行抗炎机制研究,发现paeonilactone B能抑制LPS激活的巨噬细胞中细胞因子IL-1β、IL-6、IL-18和TNF-α的分泌和炎症相关蛋白酶iNOS和COX-2的生成,同时,能抑制信号通路蛋白(JNK、ERK1/2、p38MAPK、IκBα、NF-κB/p65)的磷酸化;另外,paeonilactone B对流感病毒A/WSN/33/2009 H1N1(WSN)感染的巨噬细胞中的NO、TNF-α和IL-18产生也具有抑制作用,同时降低iNOS和COX-2的表达,但对IL-1β、IL-6的分泌没有影响.实验结果表明:paeonilactone B在LPS激活的巨噬细胞中可以通过抑制MAPKs和NF-κB信号通路来降低炎症因子的分泌而发挥抗炎活性;此外,对流感病毒感染的巨噬细胞也表现出一定的抗炎潜力.This study investigates the anti-inflammatory activity and mechanism of paeonilactone B found in the traditional Chinese medicine Paeoniae Radix Rubra in vitro.An in vitro anti-inflammatory model of RAW264.7 macrophages induced by lipopolysaccharide(LPS)was established.It was found that paeonilactone B significantly inhibited the production ofnitric oxide(NO)in LPS induced macrophages with IC 50 value of 6.09±1.81μmol/L.The anti-inflammatory mechanism research showed that paeonilactone B inhibited the secretion of cytokines IL-1β,IL-6,IL-18 and TNF-αand reduced the expression of iNOS and COX-2 on LPS-induced macrophages.Additionally,it suppressed the phosphorylation of signaling pathway proteins(JNK,ERK1/2,p38MAPK,IκBα,NF-κB/p65).Furthermore,paeonilactone B also decreased the level of NO,TNF-α,IL-18 and the expression of iNOS and COX-2 in macrophages infected with influenza virus A/WSN/33/2009 H1N1(WSN),but had no effect on IL-1βand IL-6.The results indicate that paeonilactone B exerts its anti-inflammatory activity in LPS-activated macrophages by inhibiting MAPKs and NF-κB signaling pathways to reduce the secretion of inflammatory factors.Additionally,it demonstrates potential anti-inflammatory effects in macrophages infected with the influenza virus.

关 键 词：paeonilactone B 巨噬细胞 抗炎活性 MAPKS NF-ΚB 

分 类 号：R285[医药卫生—中药学]